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- W2005804907 abstract "A “turn off–on” switch mode was established by using the interaction between acridine orange (AO) and DNA as an input signal and using the room temperature phosphorescence (RTP) reversible change of 3-mercaptopropionic acid (MPA)-capped Mn-doped ZnS quantum dots (QDs) as an output signal in biological fluids. AO was absorbed into the surface of Mn-doped ZnS QDs via electrostatic attraction and, thus, formed a ground-state complex through photoinduced electron transfer (PIET). This complex quenched the phosphorescence of Mn-doped ZnS QDs and then rendered the system into the “turn-off” mode. Along with the addition of DNA and embedded binding with DNA, AO was competitively induced to fall off from the surface of Mn-doped ZnS QDs and embed into the double helix structure of DNA. As a result, the RTP of Mn-doped ZnS QDs was recovered and the system consequently was rendered into “turn-on” mode. In this case, a new biosensor for DNA detection was built and has a detection limit of 0.033 mg L−1 and a detection range from 0.033 to 20 mg L−1. What is more, this kind of biosensor does not require complex pretreatments and is free from the interference from autofluorescence and scattering light. Thus, this biosensor can be used to detect DNA in biological fluids." @default.
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- W2005804907 date "2015-04-01" @default.
- W2005804907 modified "2023-09-23" @default.
- W2005804907 title "“Turn off–on” phosphorescent biosensors for detection of DNA based on quantum dots/acridine orange" @default.
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- W2005804907 doi "https://doi.org/10.1016/j.ab.2015.01.011" @default.
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