FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005821851> ?p ?o ?g. }

• W2005821851 abstract "Pulsed-field gel electrophoresis has been applied to separate DNA from mouse L1210 cells exposed to X-ray doses of 1 to 50 Gy. Simultaneous separation of marker chromosomes in the range 0.1 to 12.6 Mbp allowed calculation of the size distribution of the radiation-induced fragments. The distribution was consistent with a random induction of double-strand breaks (DSBs). A theoretical relationship between the size distribution of such fragments and the average number of induced breaks was used to calculate the yield and dose response. The DNA distribution was determined by both radiolabeling and fluorescence staining. Two independent methods were used to evaluate the radiation-induced yield of DSBs, both assuming that all DNA is broken at random. In the first method we compared the theoretical and experimental fraction of DNA that is below a given size limit. By this method we estimated the yield to be 0.006-0.007 DSB/Gy per million base pairs using the radiolabel and 0.004-0.008 DSB/Gy per million base pairs by fluorescence staining. The dose response was linear in both cases. In the second method we looked only at the size distribution in the resolving part of the gel and compared it to the theoretical distribution. By this method a value of approximately 0.012 DSB/Gy/Mbp was found, using fluorescence as a measure of DNA distribution. In a normal diploid mammalian genome of size 6000 Mbp, this is equivalent to a yield of 25-50 DSBs/Gy or 70 DSBs/Gy, respectively. The second approach, which looks only at the smaller fragments, may overestimate the yield, while the first approach suffers from uncertainties about the fraction of DNA irreversibly trapped in the well. The assay has the capacity to detect a dose of less than 1 Gy." @default.
• W2005821851 created "2016-06-24" @default.
• W2005821851 creator A5011277633 @default.
• W2005821851 creator A5026182786 @default.
• W2005821851 date "1995-05-01" @default.
• W2005821851 modified "2023-10-03" @default.
• W2005821851 title "Linear Induction of DNA Double-Strand Breakage with X-Ray Dose, as Determined from DNA Fragment Size Distribution" @default.
• W2005821851 cites W1518821520 @default.
• W2005821851 cites W1604458367 @default.
• W2005821851 cites W1754109927 @default.
• W2005821851 cites W1894358280 @default.
• W2005821851 cites W1968352315 @default.
• W2005821851 cites W1968937273 @default.
• W2005821851 cites W1980697196 @default.
• W2005821851 cites W1980722928 @default.
• W2005821851 cites W1981209159 @default.
• W2005821851 cites W1982221379 @default.
• W2005821851 cites W1983049633 @default.
• W2005821851 cites W1984486705 @default.
• W2005821851 cites W1988464917 @default.
• W2005821851 cites W1991104479 @default.
• W2005821851 cites W1992544969 @default.
• W2005821851 cites W1992784048 @default.
• W2005821851 cites W1995261831 @default.
• W2005821851 cites W1998372019 @default.
• W2005821851 cites W2001175810 @default.
• W2005821851 cites W2004498595 @default.
• W2005821851 cites W2007491658 @default.
• W2005821851 cites W2013031696 @default.
• W2005821851 cites W2013404388 @default.
• W2005821851 cites W2014357164 @default.
• W2005821851 cites W2016908381 @default.
• W2005821851 cites W2022915148 @default.
• W2005821851 cites W2029611498 @default.
• W2005821851 cites W2038889579 @default.
• W2005821851 cites W2041432932 @default.
• W2005821851 cites W2042890686 @default.
• W2005821851 cites W2043119321 @default.
• W2005821851 cites W2062456916 @default.
• W2005821851 cites W2065129459 @default.
• W2005821851 cites W2069914850 @default.
• W2005821851 cites W2071287201 @default.
• W2005821851 cites W2075602336 @default.
• W2005821851 cites W2077757406 @default.
• W2005821851 cites W2086239383 @default.
• W2005821851 cites W2090242035 @default.
• W2005821851 cites W2097085176 @default.
• W2005821851 cites W2118436341 @default.
• W2005821851 cites W2128139335 @default.
• W2005821851 cites W2129191857 @default.
• W2005821851 cites W2135677568 @default.
• W2005821851 cites W2138460414 @default.
• W2005821851 cites W2140186362 @default.
• W2005821851 cites W2141853256 @default.
• W2005821851 cites W2147515565 @default.
• W2005821851 cites W2171262669 @default.
• W2005821851 cites W2202253706 @default.
• W2005821851 cites W2324597012 @default.
• W2005821851 cites W2325703235 @default.
• W2005821851 cites W2333018166 @default.
• W2005821851 doi "https://doi.org/10.2307/3579023" @default.
• W2005821851 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7724729" @default.
• W2005821851 hasPublicationYear "1995" @default.
• W2005821851 type Work @default.
• W2005821851 sameAs 2005821851 @default.
• W2005821851 citedByCount "35" @default.
• W2005821851 countsByYear W20058218512012 @default.
• W2005821851 countsByYear W20058218512016 @default.
• W2005821851 countsByYear W20058218512018 @default.
• W2005821851 crossrefType "journal-article" @default.
• W2005821851 hasAuthorship W2005821851A5011277633 @default.
• W2005821851 hasAuthorship W2005821851A5026182786 @default.
• W2005821851 hasConcept C110121322 @default.
• W2005821851 hasConcept C116084860 @default.
• W2005821851 hasConcept C120665830 @default.
• W2005821851 hasConcept C121332964 @default.
• W2005821851 hasConcept C12554922 @default.
• W2005821851 hasConcept C134306372 @default.
• W2005821851 hasConcept C153911025 @default.
• W2005821851 hasConcept C185581394 @default.
• W2005821851 hasConcept C185592680 @default.
• W2005821851 hasConcept C33923547 @default.
• W2005821851 hasConcept C54355233 @default.
• W2005821851 hasConcept C552990157 @default.
• W2005821851 hasConcept C74864618 @default.
• W2005821851 hasConcept C86803240 @default.
• W2005821851 hasConcept C91881484 @default.
• W2005821851 hasConceptScore W2005821851C110121322 @default.
• W2005821851 hasConceptScore W2005821851C116084860 @default.
• W2005821851 hasConceptScore W2005821851C120665830 @default.
• W2005821851 hasConceptScore W2005821851C121332964 @default.
• W2005821851 hasConceptScore W2005821851C12554922 @default.
• W2005821851 hasConceptScore W2005821851C134306372 @default.
• W2005821851 hasConceptScore W2005821851C153911025 @default.
• W2005821851 hasConceptScore W2005821851C185581394 @default.
• W2005821851 hasConceptScore W2005821851C185592680 @default.
• W2005821851 hasConceptScore W2005821851C33923547 @default.
• W2005821851 hasConceptScore W2005821851C54355233 @default.
• W2005821851 hasConceptScore W2005821851C552990157 @default.
• W2005821851 hasConceptScore W2005821851C74864618 @default.

• next