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- W2005846174 abstract "Three phase partitioning, a technique described for protein purification, has been employed for precipitation and purification of three different commercial preparations of alginates. Three phase partitioning works by the addition of t-butanol to aqueous solution of the polymer containing 20–30% ammonium sulphate (w/v). Three phases formed are: upper t-butanol layer, interfacial polymer precipitate and lower aqueous phase. In all the three cases, the process optimization was carried out by varying ammonium sulphate concentration, volume of t-butanol, alginate concentration and temperature. Fluorescence spectroscopy was used to show that repeated cycles of TPP also resulted in considerable reduction in polyphenol content of a crude alginate preparation." @default.
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- W2005846174 title "Three phase partitioning of carbohydrate polymers: separation and purification of alginates" @default.
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- W2005846174 doi "https://doi.org/10.1016/s0144-8617(01)00313-7" @default.
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