FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005847047> ?p ?o ?g. }

• W2005847047 endingPage "1337" @default.
• W2005847047 startingPage "1331" @default.
• W2005847047 abstract "Little is known about the mechanism(s) of endothelial dysfunction in diabetes. In this study, the effect of nonenzymatic glycated LDL, a phenomenon induced by elevated D-glucose levels associated with diabetes, on porcine aortic endothelial cells was investigated. Two fractions of LDL from diabetic patients were separated by affinity column chromatography and are referred to herein as fraction alpha (nonglycated LDL) and fraction beta (glycated LDL). Incubation of endothelial cells for 24 h with total LDL isolated from diabetic subjects (dLDL) increased the release of superoxide anions (*O2-) by fivefold, while no effect of LDL isolated from healthy individuals (nLDL) was found. Fraction beta, but not fraction alpha, evoked the *O2- release. In vitro-glycated LDL mimicked the effect of dLDL/fraction beta on *O2- release that correlated with its degree of glycation (R2 = 0.96). Moreover, nitric oxide (NO) stability (measured with a porphyrinic-based electrode) and NO bioactivity (measured by its ability to elevate cellular cGMP levels) were reduced in cells treated with dLDL by 46 and 41%, respectively. dLDL (but not nLDL or fraction alpha) abolished shear stress-induced L-arginine uptake. The inhibitory effect of dLDL on shear stress-induced L-arginine uptake was mimicked by in vitro-glycated LDL. The efficiency of in vitro-glycated LDL to diminish shear stress-evoked L-arginine uptake correlated with the extent of glycation (R2 = 0.88). Moreover, dLDL, but not nLDL or fraction alpha, reduced shear stress-mediated cGMP formation and NOx production by 47 and 88%, respectively. This effect was also mimicked by in vitro-glycated LDL, correlating with its degree of glycation (R2 = 0.86). Under these experimental conditions, glycated LDL reduced shear stress-induced increase in NO synthesis by inhibition of shear stress-stimulated L-arginine uptake and NO bioactivity due to increased endothelial cell *O2- release. These properties may contribute to the reduced vasodilatory response and the vascular complications in diabetes." @default.
• W2005847047 created "2016-06-24" @default.
• W2005847047 creator A5004971733 @default.
• W2005847047 creator A5021827648 @default.
• W2005847047 creator A5024537624 @default.
• W2005847047 creator A5029076692 @default.
• W2005847047 creator A5035066978 @default.
• W2005847047 creator A5053111848 @default.
• W2005847047 creator A5056239518 @default.
• W2005847047 date "1999-06-01" @default.
• W2005847047 modified "2023-10-15" @default.
• W2005847047 title "Glycated low-density lipoprotein attenuates shear stress-induced nitric oxide synthesis by inhibition of shear stress-activated L-arginine uptake in endothelial cells." @default.
• W2005847047 cites W1553328291 @default.
• W2005847047 cites W1834350010 @default.
• W2005847047 cites W1970728146 @default.
• W2005847047 cites W1991091544 @default.
• W2005847047 cites W1993114150 @default.
• W2005847047 cites W1993310669 @default.
• W2005847047 cites W2013443695 @default.
• W2005847047 cites W2025458248 @default.
• W2005847047 cites W2032278674 @default.
• W2005847047 cites W2038455348 @default.
• W2005847047 cites W2046382167 @default.
• W2005847047 cites W2059720805 @default.
• W2005847047 cites W2089336066 @default.
• W2005847047 cites W2096281503 @default.
• W2005847047 cites W2114295374 @default.
• W2005847047 cites W2123306608 @default.
• W2005847047 doi "https://doi.org/10.2337/diabetes.48.6.1331" @default.
• W2005847047 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10342824" @default.
• W2005847047 hasPublicationYear "1999" @default.
• W2005847047 type Work @default.
• W2005847047 sameAs 2005847047 @default.
• W2005847047 citedByCount "53" @default.
• W2005847047 countsByYear W20058470472013 @default.
• W2005847047 countsByYear W20058470472014 @default.
• W2005847047 countsByYear W20058470472015 @default.
• W2005847047 countsByYear W20058470472016 @default.
• W2005847047 countsByYear W20058470472017 @default.
• W2005847047 countsByYear W20058470472019 @default.
• W2005847047 countsByYear W20058470472021 @default.
• W2005847047 countsByYear W20058470472022 @default.
• W2005847047 countsByYear W20058470472023 @default.
• W2005847047 crossrefType "journal-article" @default.
• W2005847047 hasAuthorship W2005847047A5004971733 @default.
• W2005847047 hasAuthorship W2005847047A5021827648 @default.
• W2005847047 hasAuthorship W2005847047A5024537624 @default.
• W2005847047 hasAuthorship W2005847047A5029076692 @default.
• W2005847047 hasAuthorship W2005847047A5035066978 @default.
• W2005847047 hasAuthorship W2005847047A5053111848 @default.
• W2005847047 hasAuthorship W2005847047A5056239518 @default.
• W2005847047 hasConcept C12554922 @default.
• W2005847047 hasConcept C126322002 @default.
• W2005847047 hasConcept C134018914 @default.
• W2005847047 hasConcept C147990577 @default.
• W2005847047 hasConcept C159985019 @default.
• W2005847047 hasConcept C170493617 @default.
• W2005847047 hasConcept C185592680 @default.
• W2005847047 hasConcept C192562407 @default.
• W2005847047 hasConcept C202751555 @default.
• W2005847047 hasConcept C21141959 @default.
• W2005847047 hasConcept C25642318 @default.
• W2005847047 hasConcept C2776992346 @default.
• W2005847047 hasConcept C2777468819 @default.
• W2005847047 hasConcept C2780972559 @default.
• W2005847047 hasConcept C515207424 @default.
• W2005847047 hasConcept C519581460 @default.
• W2005847047 hasConcept C55493867 @default.
• W2005847047 hasConcept C71924100 @default.
• W2005847047 hasConcept C86803240 @default.
• W2005847047 hasConceptScore W2005847047C12554922 @default.
• W2005847047 hasConceptScore W2005847047C126322002 @default.
• W2005847047 hasConceptScore W2005847047C134018914 @default.
• W2005847047 hasConceptScore W2005847047C147990577 @default.
• W2005847047 hasConceptScore W2005847047C159985019 @default.
• W2005847047 hasConceptScore W2005847047C170493617 @default.
• W2005847047 hasConceptScore W2005847047C185592680 @default.
• W2005847047 hasConceptScore W2005847047C192562407 @default.
• W2005847047 hasConceptScore W2005847047C202751555 @default.
• W2005847047 hasConceptScore W2005847047C21141959 @default.
• W2005847047 hasConceptScore W2005847047C25642318 @default.
• W2005847047 hasConceptScore W2005847047C2776992346 @default.
• W2005847047 hasConceptScore W2005847047C2777468819 @default.
• W2005847047 hasConceptScore W2005847047C2780972559 @default.
• W2005847047 hasConceptScore W2005847047C515207424 @default.
• W2005847047 hasConceptScore W2005847047C519581460 @default.
• W2005847047 hasConceptScore W2005847047C55493867 @default.
• W2005847047 hasConceptScore W2005847047C71924100 @default.
• W2005847047 hasConceptScore W2005847047C86803240 @default.
• W2005847047 hasIssue "6" @default.
• W2005847047 hasLocation W20058470471 @default.
• W2005847047 hasLocation W20058470472 @default.
• W2005847047 hasOpenAccess W2005847047 @default.
• W2005847047 hasPrimaryLocation W20058470471 @default.
• W2005847047 hasRelatedWork W1607531870 @default.
• W2005847047 hasRelatedWork W2022809694 @default.
• W2005847047 hasRelatedWork W2025791617 @default.
• W2005847047 hasRelatedWork W2028770962 @default.

• next