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• W2005885353 abstract "No AccessJournal of UrologyInvestigative Urology1 May 2012Two-Layer Tissue Engineered Urethra Using Oral Epithelial and Muscle Derived Cells Hiroshi Mikami, Go Kuwahara, Nobuyuki Nakamura, Masayuki Yamato, Masatoshi Tanaka, and Shohta Kodama Hiroshi MikamiHiroshi Mikami Department of Urology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan Department of Regenerative Medicine and Transplantation, Faculty of Medicine, Fukuoka University, Fukuoka, Japan More articles by this author , Go KuwaharaGo Kuwahara Department of Regenerative Medicine and Transplantation, Faculty of Medicine, Fukuoka University, Fukuoka, Japan More articles by this author , Nobuyuki NakamuraNobuyuki Nakamura Department of Urology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan More articles by this author , Masayuki YamatoMasayuki Yamato Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan More articles by this author , Masatoshi TanakaMasatoshi Tanaka Department of Urology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan More articles by this author , and Shohta KodamaShohta Kodama Department of Regenerative Medicine and Transplantation, Faculty of Medicine, Fukuoka University, Fukuoka, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.12.059AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We fabricated novel tissue engineered urethral grafts using autologously harvested oral cells. We report their viability in a canine model. Materials and Methods: Oral tissues were harvested by punch biopsy and divided into mucosal and muscle sections. Epithelial cells from mucosal sections were cultured as epithelial cell sheets. Simultaneously muscle derived cells were seeded on collagen mesh matrices to form muscle cell sheets. At 2 weeks the sheets were joined and tubularized to form 2-layer tissue engineered urethras, which were autologously grafted to surgically induced urethral defects in 10 dogs in the experimental group. Tissue engineered grafts were not applied to the induced urethral defect in control dogs. The dogs were followed 12 weeks postoperatively. Urethrogram and histological examination were done to evaluate the grafting outcome. Results: We successfully fabricated 2-layer tissue engineered urethras in vitro and transplanted them in dogs in the experimental group. The 12-week complication-free rate was significantly higher in the experimental group than in controls. Urethrogram confirmed urethral patency without stricture in the complication-free group at 12 weeks. Histologically urethras in the transplant group showed a stratified epithelial layer overlying well differentiated submucosa. In contrast, urethras in controls showed severe fibrosis without epithelial layer formation. Conclusions: Two-layer tissue engineered urethras were engineered using cells harvested by minimally invasive oral punch biopsy. Results suggest that this technique can encourage regeneration of a functional urethra. References 1 : Buccal mucosal grafts for urethral reconstruction. Urology1998; 51: 15. Google Scholar 2 : Substitution urethroplasty with buccal mucosal-free grafts. J Urol2001; 165: 1131. Link, Google Scholar 3 : Buccal mucosal urethroplasty: is it the new gold standard?. BJU Int2004; 93: 1191. 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Google Scholar © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 5May 2012Page: 1882-1889 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.Keywordstissue engineeringdogsurethratransplantsepithelial cellsAcknowledgmentsAtree, Tokyo, Japan, provided collagen mesh matrices.MetricsAuthor Information Hiroshi Mikami Department of Urology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan Department of Regenerative Medicine and Transplantation, Faculty of Medicine, Fukuoka University, Fukuoka, Japan More articles by this author Go Kuwahara Department of Regenerative Medicine and Transplantation, Faculty of Medicine, Fukuoka University, Fukuoka, Japan More articles by this author Nobuyuki Nakamura Department of Urology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan More articles by this author Masayuki Yamato Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan More articles by this author Masatoshi Tanaka Department of Urology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan More articles by this author Shohta Kodama Department of Regenerative Medicine and Transplantation, Faculty of Medicine, Fukuoka University, Fukuoka, Japan More articles by this author Expand All Advertisement PDF downloadLoading ..." @default.
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• W2005885353 title "Two-Layer Tissue Engineered Urethra Using Oral Epithelial and Muscle Derived Cells" @default.
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