FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005975860> ?p ?o ?g. }

• W2005975860 endingPage "4311" @default.
• W2005975860 startingPage "4306" @default.
• W2005975860 abstract "The ubiquitination of proteins can signal their degradation, modify their activity or target them to specific membranes or cellular organelles. Here, we show that monoubiquitination regulates the plasma membrane abundance and function of the potassium channel, ROMK. Immunoprecipitation of proteins obtained from renal cortex and outer medulla with ROMK antibody revealed that this channel was monoubiquitinated. To determine the ubiquitin binding site on ROMK1, all intracellular lysine (Lys) residues of ROMK1 were individually mutated to arginine (Arg), and a two-electrode voltage clamp was used to measure the ROMK1 channel activity in Xenopus oocytes. ROMK1 channel activity increased from 8.1 to 27.2 μA only when Lys-22 was mutated to Arg. Furthermore, Western blotting failed to detect the ubiquitinated ROMK1 in oocytes injected with R1K22R. Patch-clamp experiments showed that biophysical properties of R1K22R were identical to those of wild-type ROMK1. Although total protein expression levels of GFP-ROMK1 and GFP-R1K22R in oocytes were similar, confocal microscopy showed that the surface fluorescence intensity in oocytes injected with GFP-R1K22R was higher than that of GFP-ROMK1. In addition, biotin labeling of ROMK1 and R1K22R proteins expressed in HEK293 cells showed increased surface expression of the Lys-22 mutant channel. Finally, expression of R1K22R in COS7 cells significantly stimulated the surface expression of ROMK1. We conclude that ROMK1 can be monoubiquitinated and that Lys-22 is an ubiquitin-binding site. Thus, monoubiquitination of ROMK1 regulates channel activity by reducing the surface expression of channel protein. This finding implicates the linking of a single ubiquitin molecule to channels as an important posttranslational regulatory signal." @default.
• W2005975860 created "2016-06-24" @default.
• W2005975860 creator A5021311035 @default.
• W2005975860 creator A5029871548 @default.
• W2005975860 creator A5031782903 @default.
• W2005975860 creator A5032533121 @default.
• W2005975860 creator A5054544222 @default.
• W2005975860 creator A5074744340 @default.
• W2005975860 creator A5077196517 @default.
• W2005975860 creator A5085236573 @default.
• W2005975860 creator A5085876043 @default.
• W2005975860 date "2005-03-14" @default.
• W2005975860 modified "2023-09-29" @default.
• W2005975860 title "ROMK1 channel activity is regulated by monoubiquitination" @default.
• W2005975860 cites W1494477854 @default.
• W2005975860 cites W1967391569 @default.
• W2005975860 cites W1976635506 @default.
• W2005975860 cites W1979274256 @default.
• W2005975860 cites W1987514645 @default.
• W2005975860 cites W1993454528 @default.
• W2005975860 cites W1995826486 @default.
• W2005975860 cites W1998790265 @default.
• W2005975860 cites W2002223631 @default.
• W2005975860 cites W2006423155 @default.
• W2005975860 cites W2024812887 @default.
• W2005975860 cites W2036644816 @default.
• W2005975860 cites W2052779687 @default.
• W2005975860 cites W2053049178 @default.
• W2005975860 cites W2055786440 @default.
• W2005975860 cites W2060458509 @default.
• W2005975860 cites W2081500927 @default.
• W2005975860 cites W2085202207 @default.
• W2005975860 cites W2094866737 @default.
• W2005975860 cites W2096675347 @default.
• W2005975860 cites W2102064219 @default.
• W2005975860 cites W2107609623 @default.
• W2005975860 cites W2110524964 @default.
• W2005975860 cites W2121091466 @default.
• W2005975860 cites W2126561023 @default.
• W2005975860 cites W2131350125 @default.
• W2005975860 cites W2133987610 @default.
• W2005975860 cites W2134168892 @default.
• W2005975860 cites W2135982229 @default.
• W2005975860 cites W2140853270 @default.
• W2005975860 cites W2153991959 @default.
• W2005975860 cites W2159150139 @default.
• W2005975860 cites W2167740142 @default.
• W2005975860 cites W2179236258 @default.
• W2005975860 cites W2182749815 @default.
• W2005975860 cites W2183139538 @default.
• W2005975860 cites W2194462879 @default.
• W2005975860 cites W2211384723 @default.
• W2005975860 cites W2214123781 @default.
• W2005975860 cites W2265391412 @default.
• W2005975860 cites W2299765754 @default.
• W2005975860 cites W2408893534 @default.
• W2005975860 cites W2413431366 @default.
• W2005975860 doi "https://doi.org/10.1073/pnas.0409767102" @default.
• W2005975860 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/555508" @default.
• W2005975860 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15767585" @default.
• W2005975860 hasPublicationYear "2005" @default.
• W2005975860 type Work @default.
• W2005975860 sameAs 2005975860 @default.
• W2005975860 citedByCount "44" @default.
• W2005975860 countsByYear W20059758602013 @default.
• W2005975860 countsByYear W20059758602015 @default.
• W2005975860 countsByYear W20059758602016 @default.
• W2005975860 countsByYear W20059758602018 @default.
• W2005975860 countsByYear W20059758602019 @default.
• W2005975860 countsByYear W20059758602020 @default.
• W2005975860 countsByYear W20059758602021 @default.
• W2005975860 countsByYear W20059758602022 @default.
• W2005975860 countsByYear W20059758602023 @default.
• W2005975860 crossrefType "journal-article" @default.
• W2005975860 hasAuthorship W2005975860A5021311035 @default.
• W2005975860 hasAuthorship W2005975860A5029871548 @default.
• W2005975860 hasAuthorship W2005975860A5031782903 @default.
• W2005975860 hasAuthorship W2005975860A5032533121 @default.
• W2005975860 hasAuthorship W2005975860A5054544222 @default.
• W2005975860 hasAuthorship W2005975860A5074744340 @default.
• W2005975860 hasAuthorship W2005975860A5077196517 @default.
• W2005975860 hasAuthorship W2005975860A5085236573 @default.
• W2005975860 hasAuthorship W2005975860A5085876043 @default.
• W2005975860 hasBestOaLocation W20059758602 @default.
• W2005975860 hasConcept C104317684 @default.
• W2005975860 hasConcept C12554922 @default.
• W2005975860 hasConcept C142613039 @default.
• W2005975860 hasConcept C147944092 @default.
• W2005975860 hasConcept C153911025 @default.
• W2005975860 hasConcept C170493617 @default.
• W2005975860 hasConcept C174510640 @default.
• W2005975860 hasConcept C185592680 @default.
• W2005975860 hasConcept C25602115 @default.
• W2005975860 hasConcept C2779535977 @default.
• W2005975860 hasConcept C55493867 @default.
• W2005975860 hasConcept C71829478 @default.
• W2005975860 hasConcept C83743174 @default.
• W2005975860 hasConcept C86803240 @default.

• next