Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005991921> ?p ?o ?g. }
- W2005991921 endingPage "283" @default.
- W2005991921 startingPage "276" @default.
- W2005991921 abstract "In view of the wide usage of frozen PBMCs as stem cell support following high-dose chemo- and/or radiotherapy, and the pleiotropic activities of tumor necrosis factor-alpha (TNF-alpha), the influences of freezing and radiation on LPS-induced TNF-alpha production by human peripheral blood mononuclear cells (PBMCs) were studied. Frozen PBMCs secreted significantly larger quantities of TNF-alpha than fresh cells. Blocking of endogenous IL-10 by neutralization with anti-IL-10 monoclonal antibody resulted in further augmented and prolonged secretion of TNF-alpha by both the fresh and frozen cells. In contrast, addition of exogenous IL-10 to LPS-stimulated cultures inhibited TNF-alpha secretion. In vitro irradiation had an inconsistent effect on TNF-alpha production by the fresh PBMCs. Taken together, these results suggest that the endogenously hypersecreted TNF-alpha is indirectly responsible for the previously reported elevated IL-1-, IL-6-, and IL-10-secreting capabilities of frozen PBMCs. They also indicate that the TNF-alpha induced IL-10 and then down-regulates the monocytes from further TNF-alpha secretion. Considering the vital role played by TNF-alpha in antimicrobial and antitumor activities, in the immune system, and in the pathogenesis of many acute and chronic diseases, the abilities of frozen cells to produce large quantities of TNF-alpha in response to infectious agents could have profound impact on patients receiving such frozen PBMCs as stem cell support following myeloablative therapies." @default.
- W2005991921 created "2016-06-24" @default.
- W2005991921 creator A5084626313 @default.
- W2005991921 date "1997-05-01" @default.
- W2005991921 modified "2023-09-26" @default.
- W2005991921 title "Effects of Cryopreservation on Immune Responses" @default.
- W2005991921 cites W1499394053 @default.
- W2005991921 cites W1529226217 @default.
- W2005991921 cites W1557653296 @default.
- W2005991921 cites W162868008 @default.
- W2005991921 cites W1678649710 @default.
- W2005991921 cites W1713837849 @default.
- W2005991921 cites W180289105 @default.
- W2005991921 cites W1981696952 @default.
- W2005991921 cites W1988096834 @default.
- W2005991921 cites W2002209097 @default.
- W2005991921 cites W2003514161 @default.
- W2005991921 cites W2016951403 @default.
- W2005991921 cites W2023022687 @default.
- W2005991921 cites W2031674167 @default.
- W2005991921 cites W2053625920 @default.
- W2005991921 cites W2057123987 @default.
- W2005991921 cites W2070737639 @default.
- W2005991921 cites W2078913105 @default.
- W2005991921 cites W2081166085 @default.
- W2005991921 cites W2122462682 @default.
- W2005991921 cites W2126342903 @default.
- W2005991921 cites W2136255098 @default.
- W2005991921 cites W2142585662 @default.
- W2005991921 cites W2142879848 @default.
- W2005991921 cites W2144152742 @default.
- W2005991921 cites W2176899369 @default.
- W2005991921 cites W2185577508 @default.
- W2005991921 cites W2191654227 @default.
- W2005991921 cites W2322913198 @default.
- W2005991921 doi "https://doi.org/10.1006/cryo.1997.2005" @default.
- W2005991921 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9160997" @default.
- W2005991921 hasPublicationYear "1997" @default.
- W2005991921 type Work @default.
- W2005991921 sameAs 2005991921 @default.
- W2005991921 citedByCount "10" @default.
- W2005991921 countsByYear W20059919212012 @default.
- W2005991921 countsByYear W20059919212015 @default.
- W2005991921 countsByYear W20059919212018 @default.
- W2005991921 countsByYear W20059919212019 @default.
- W2005991921 countsByYear W20059919212022 @default.
- W2005991921 crossrefType "journal-article" @default.
- W2005991921 hasAuthorship W2005991921A5084626313 @default.
- W2005991921 hasConcept C134018914 @default.
- W2005991921 hasConcept C137061746 @default.
- W2005991921 hasConcept C159654299 @default.
- W2005991921 hasConcept C16613235 @default.
- W2005991921 hasConcept C17991360 @default.
- W2005991921 hasConcept C179933525 @default.
- W2005991921 hasConcept C196843134 @default.
- W2005991921 hasConcept C202751555 @default.
- W2005991921 hasConcept C203014093 @default.
- W2005991921 hasConcept C2778690821 @default.
- W2005991921 hasConcept C49039625 @default.
- W2005991921 hasConcept C542903549 @default.
- W2005991921 hasConcept C55493867 @default.
- W2005991921 hasConcept C71924100 @default.
- W2005991921 hasConcept C86803240 @default.
- W2005991921 hasConcept C8891405 @default.
- W2005991921 hasConcept C95444343 @default.
- W2005991921 hasConceptScore W2005991921C134018914 @default.
- W2005991921 hasConceptScore W2005991921C137061746 @default.
- W2005991921 hasConceptScore W2005991921C159654299 @default.
- W2005991921 hasConceptScore W2005991921C16613235 @default.
- W2005991921 hasConceptScore W2005991921C17991360 @default.
- W2005991921 hasConceptScore W2005991921C179933525 @default.
- W2005991921 hasConceptScore W2005991921C196843134 @default.
- W2005991921 hasConceptScore W2005991921C202751555 @default.
- W2005991921 hasConceptScore W2005991921C203014093 @default.
- W2005991921 hasConceptScore W2005991921C2778690821 @default.
- W2005991921 hasConceptScore W2005991921C49039625 @default.
- W2005991921 hasConceptScore W2005991921C542903549 @default.
- W2005991921 hasConceptScore W2005991921C55493867 @default.
- W2005991921 hasConceptScore W2005991921C71924100 @default.
- W2005991921 hasConceptScore W2005991921C86803240 @default.
- W2005991921 hasConceptScore W2005991921C8891405 @default.
- W2005991921 hasConceptScore W2005991921C95444343 @default.
- W2005991921 hasIssue "3" @default.
- W2005991921 hasLocation W20059919211 @default.
- W2005991921 hasLocation W20059919212 @default.
- W2005991921 hasOpenAccess W2005991921 @default.
- W2005991921 hasPrimaryLocation W20059919211 @default.
- W2005991921 hasRelatedWork W1705661556 @default.
- W2005991921 hasRelatedWork W2000373946 @default.
- W2005991921 hasRelatedWork W2009244638 @default.
- W2005991921 hasRelatedWork W2029372981 @default.
- W2005991921 hasRelatedWork W2047209980 @default.
- W2005991921 hasRelatedWork W2085322992 @default.
- W2005991921 hasRelatedWork W2087976276 @default.
- W2005991921 hasRelatedWork W2113148377 @default.
- W2005991921 hasRelatedWork W2369676305 @default.
- W2005991921 hasRelatedWork W9581748 @default.
- W2005991921 hasVolume "34" @default.