FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2006001463> ?p ?o ?g. }

• W2006001463 endingPage "e31789" @default.
• W2006001463 startingPage "e31789" @default.
• W2006001463 abstract "This study uses YFP-tagged Rab27b expression in rabbit lacrimal gland acinar cells, which are polarized secretory epithelial cells, to characterize early stages of secretory vesicle trafficking. Here we demonstrate the utility of YFP-Rab27b to delineate new perspectives on the mechanisms of early vesicle biogenesis in lacrimal gland acinar cells, where information is significantly limited. Protocols were developed to deplete the mature YFP-Rab27b-enriched secretory vesicle pool in the subapical region of the cell, and confocal fluorescence microscopy was used to track vesicle replenishment. This analysis revealed a basally-localized organelle, which we termed the nascent vesicle site, from which nascent vesicles appeared to emerge. Subapical vesicular YFP-Rab27b was co-localized with p150(Glued), a component of the dynactin cofactor of cytoplasmic dynein. Treatment with the microtubule-targeted agent, nocodazole, did not affect release of mature secretory vesicles, although during vesicle repletion it significantly altered nascent YFP-Rab27b-enriched secretory vesicle localization. Instead of moving to the subapical region, these vesicles were trapped at the nascent vesicle site which was adjacent to, if not a sub-compartment of, the trans-Golgi network. Finally, YFP-Rab27b-enriched secretory vesicles which reached the subapical cytoplasm appeared to acquire the actin-based motor protein, Myosin 5C. Our findings show that Rab27b enrichment occurs early in secretory vesicle formation, that secretory vesicles bud from a visually discernable nascent vesicle site, and that transport from the nascent vesicle site to the subapical region requires intact microtubules." @default.
• W2006001463 created "2016-06-24" @default.
• W2006001463 creator A5005250451 @default.
• W2006001463 creator A5040824763 @default.
• W2006001463 creator A5059857634 @default.
• W2006001463 date "2012-02-20" @default.
• W2006001463 modified "2023-09-30" @default.
• W2006001463 title "Direct Imaging of RAB27B-Enriched Secretory Vesicle Biogenesis in Lacrimal Acinar Cells Reveals Origins on a Nascent Vesicle Budding Site" @default.
• W2006001463 cites W1491410831 @default.
• W2006001463 cites W1494687941 @default.
• W2006001463 cites W1535881221 @default.
• W2006001463 cites W1536788210 @default.
• W2006001463 cites W1540345360 @default.
• W2006001463 cites W1655601628 @default.
• W2006001463 cites W1839466617 @default.
• W2006001463 cites W1922265191 @default.
• W2006001463 cites W1973200579 @default.
• W2006001463 cites W1977353559 @default.
• W2006001463 cites W1978409524 @default.
• W2006001463 cites W1979217451 @default.
• W2006001463 cites W1979751563 @default.
• W2006001463 cites W1986180217 @default.
• W2006001463 cites W1990834162 @default.
• W2006001463 cites W1995497833 @default.
• W2006001463 cites W1998153400 @default.
• W2006001463 cites W1998389060 @default.
• W2006001463 cites W2007527685 @default.
• W2006001463 cites W2011172253 @default.
• W2006001463 cites W2011431692 @default.
• W2006001463 cites W2014064711 @default.
• W2006001463 cites W2016256493 @default.
• W2006001463 cites W2020269189 @default.
• W2006001463 cites W2022697316 @default.
• W2006001463 cites W2026987995 @default.
• W2006001463 cites W2031418444 @default.
• W2006001463 cites W2033757538 @default.
• W2006001463 cites W2034374571 @default.
• W2006001463 cites W2035417297 @default.
• W2006001463 cites W2036420977 @default.
• W2006001463 cites W2048837254 @default.
• W2006001463 cites W2058428900 @default.
• W2006001463 cites W2059398027 @default.
• W2006001463 cites W2064880862 @default.
• W2006001463 cites W2068358300 @default.
• W2006001463 cites W2071872742 @default.
• W2006001463 cites W2074209621 @default.
• W2006001463 cites W2077214620 @default.
• W2006001463 cites W2092834961 @default.
• W2006001463 cites W2092956427 @default.
• W2006001463 cites W2093852065 @default.
• W2006001463 cites W2097940631 @default.
• W2006001463 cites W2109126691 @default.
• W2006001463 cites W2116872337 @default.
• W2006001463 cites W2118179399 @default.
• W2006001463 cites W2118642774 @default.
• W2006001463 cites W2121031578 @default.
• W2006001463 cites W2125369555 @default.
• W2006001463 cites W2127239742 @default.
• W2006001463 cites W2128452615 @default.
• W2006001463 cites W2129118202 @default.
• W2006001463 cites W2130359731 @default.
• W2006001463 cites W2132099293 @default.
• W2006001463 cites W2134492570 @default.
• W2006001463 cites W2135736561 @default.
• W2006001463 cites W2138947981 @default.
• W2006001463 cites W2151026886 @default.
• W2006001463 cites W2160125876 @default.
• W2006001463 cites W2160797468 @default.
• W2006001463 cites W2161196100 @default.
• W2006001463 cites W2165161764 @default.
• W2006001463 cites W2167889794 @default.
• W2006001463 cites W2171403233 @default.
• W2006001463 cites W2171470705 @default.
• W2006001463 cites W2182309232 @default.
• W2006001463 cites W2193007022 @default.
• W2006001463 doi "https://doi.org/10.1371/journal.pone.0031789" @default.
• W2006001463 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3282733" @default.
• W2006001463 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22363735" @default.
• W2006001463 hasPublicationYear "2012" @default.
• W2006001463 type Work @default.
• W2006001463 sameAs 2006001463 @default.
• W2006001463 citedByCount "10" @default.
• W2006001463 countsByYear W20060014632012 @default.
• W2006001463 countsByYear W20060014632013 @default.
• W2006001463 countsByYear W20060014632015 @default.
• W2006001463 countsByYear W20060014632016 @default.
• W2006001463 countsByYear W20060014632017 @default.
• W2006001463 countsByYear W20060014632019 @default.
• W2006001463 countsByYear W20060014632022 @default.
• W2006001463 countsByYear W20060014632023 @default.
• W2006001463 crossrefType "journal-article" @default.
• W2006001463 hasAuthorship W2006001463A5005250451 @default.
• W2006001463 hasAuthorship W2006001463A5040824763 @default.
• W2006001463 hasAuthorship W2006001463A5059857634 @default.
• W2006001463 hasBestOaLocation W20060014631 @default.
• W2006001463 hasConcept C119062480 @default.
• W2006001463 hasConcept C130316041 @default.
• W2006001463 hasConcept C158617107 @default.

• next