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- W2006011202 abstract "In lineage tracing analysis, the beta-galactosidase (beta-gal) gene is a commonly used as a reporter gene because it is relatively stable and highly sensitive in histochemical detection using 5-bromo-4-chloro-3-indolyl-beta-d-galactoside (X-gal). Clear determination of the types and characteristics of labeled cells requires transmission electron microscopic (TEM) examination of their morphology. X-gal staining, which involves the precipitate formed by the reaction between beta-gal and X-gal, is usually recognized as a light blue or green reaction product on light microscopic (LM) examination. However, the standard protocol for TEM preparation weakens the intensity of or results in the loss of X-gal reaction product at the step of substitution of ethanol with Epon using propylene oxide. To solve this problem, we show that hydroxypropyl methacrylate achieves good preservation of X-gal reaction products. The protocol presented here appears to be useful for lineage determination by TEM of all types of X-gal-stained tissues." @default.
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- W2006011202 date "2005-02-01" @default.
- W2006011202 modified "2023-09-26" @default.
- W2006011202 title "Improved preservation of X-gal reaction product for electron microscopy using hydroxypropyl methacrylate" @default.
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- W2006011202 doi "https://doi.org/10.1016/j.neulet.2004.10.023" @default.
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