Matches in SemOpenAlex for { <https://semopenalex.org/work/W2006027695> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2006027695 endingPage "259" @default.
- W2006027695 startingPage "251" @default.
- W2006027695 abstract "A method for biochemically isolating microtubule-associated proteins (MAPs) from the detergent-extracted cytoskeletons of carrot suspension cells has been devised. The advantage of cytoskeletons is that filamentous proteins are enriched and separated from vacuolar contents. Depolymerization of cytoskeletal microtubules with calcium at 4°C releases MAPs which are then isolated by association with taxol stabilized neurotubules. Stripped from microtubules (MTs) by salt, then dialysed, the resulting fraction contains a limited number of high molecular weight proteins. Turbidimetric assays demonstrate that this MAP fraction stimulates polymerization of tubulin at concentrations at which it does not self-assemble. By adding it to rhodamine-conjugated tubulin, the fraction can be seen to form radiating arrays of long filaments, unlike MTs induced by taxol. In the electron microscope, these arrays are seen to be composed of mainly single microtubules. Blot-affinity purified antibodies confirm that two of the proteins decorate cellular microtubules and fulfil the criteria for MAPs. Antibodies to an antigenically related triplet of proteins about 60–68 kDa (MAP 65) stain interphase, preprophase band, spindle and phragmoplast microtubules. Antibodies to the 120 kDa MAP also stain all of the MT arrays but labelling of the cortical MTs is more punctate and, unlike anti-MAP 65, the nuclear periphery is also stained. Both the anti-65 kDa and the anti-120 kDa antibodies stain cortical MTs in detergent-extracted, substrate-attached plasma membrane disks (‘footprints’). Since the 120 kDa protein is detected at two surfaces (nucleus and plasma membrane) known to support MT growth in plants, it is hypothesized that it may function there in the attachment or nucleation of MTs." @default.
- W2006027695 created "2016-06-24" @default.
- W2006027695 creator A5017923598 @default.
- W2006027695 creator A5031878740 @default.
- W2006027695 creator A5055541484 @default.
- W2006027695 date "1996-08-01" @default.
- W2006027695 modified "2023-10-17" @default.
- W2006027695 title "Isolation of microtubule-associated proteins from carrot cytoskeletons: a 120 kDa map decorates all four microtubule arrays and the nucleus" @default.
- W2006027695 doi "https://doi.org/10.1046/j.1365-313x.1996.10020251.x" @default.
- W2006027695 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8771783" @default.
- W2006027695 hasPublicationYear "1996" @default.
- W2006027695 type Work @default.
- W2006027695 sameAs 2006027695 @default.
- W2006027695 citedByCount "84" @default.
- W2006027695 countsByYear W20060276952012 @default.
- W2006027695 countsByYear W20060276952014 @default.
- W2006027695 countsByYear W20060276952016 @default.
- W2006027695 countsByYear W20060276952018 @default.
- W2006027695 countsByYear W20060276952023 @default.
- W2006027695 crossrefType "journal-article" @default.
- W2006027695 hasAuthorship W2006027695A5017923598 @default.
- W2006027695 hasAuthorship W2006027695A5031878740 @default.
- W2006027695 hasAuthorship W2006027695A5055541484 @default.
- W2006027695 hasBestOaLocation W20060276951 @default.
- W2006027695 hasConcept C120665830 @default.
- W2006027695 hasConcept C121332964 @default.
- W2006027695 hasConcept C142669718 @default.
- W2006027695 hasConcept C1491633281 @default.
- W2006027695 hasConcept C166029249 @default.
- W2006027695 hasConcept C20418707 @default.
- W2006027695 hasConcept C55493867 @default.
- W2006027695 hasConcept C61081601 @default.
- W2006027695 hasConcept C84425145 @default.
- W2006027695 hasConcept C86803240 @default.
- W2006027695 hasConcept C93877712 @default.
- W2006027695 hasConcept C95444343 @default.
- W2006027695 hasConceptScore W2006027695C120665830 @default.
- W2006027695 hasConceptScore W2006027695C121332964 @default.
- W2006027695 hasConceptScore W2006027695C142669718 @default.
- W2006027695 hasConceptScore W2006027695C1491633281 @default.
- W2006027695 hasConceptScore W2006027695C166029249 @default.
- W2006027695 hasConceptScore W2006027695C20418707 @default.
- W2006027695 hasConceptScore W2006027695C55493867 @default.
- W2006027695 hasConceptScore W2006027695C61081601 @default.
- W2006027695 hasConceptScore W2006027695C84425145 @default.
- W2006027695 hasConceptScore W2006027695C86803240 @default.
- W2006027695 hasConceptScore W2006027695C93877712 @default.
- W2006027695 hasConceptScore W2006027695C95444343 @default.
- W2006027695 hasIssue "2" @default.
- W2006027695 hasLocation W20060276951 @default.
- W2006027695 hasLocation W20060276952 @default.
- W2006027695 hasOpenAccess W2006027695 @default.
- W2006027695 hasPrimaryLocation W20060276951 @default.
- W2006027695 hasRelatedWork W1901412126 @default.
- W2006027695 hasRelatedWork W1974089612 @default.
- W2006027695 hasRelatedWork W2024145976 @default.
- W2006027695 hasRelatedWork W2031263977 @default.
- W2006027695 hasRelatedWork W2070191397 @default.
- W2006027695 hasRelatedWork W2079016385 @default.
- W2006027695 hasRelatedWork W2107561141 @default.
- W2006027695 hasRelatedWork W2188467511 @default.
- W2006027695 hasRelatedWork W2901583425 @default.
- W2006027695 hasRelatedWork W2528453622 @default.
- W2006027695 hasVolume "10" @default.
- W2006027695 isParatext "false" @default.
- W2006027695 isRetracted "false" @default.
- W2006027695 magId "2006027695" @default.
- W2006027695 workType "article" @default.