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• W2006028144 abstract "Human FCP1 in association with RNAP II reconstitutes a highly specific CTD phosphatase activity and is required for recycling RNA polymerase II (RNAP II) in vitro. Here we demonstrate that targeted recruitment of FCP1 to promoter templates, through fusion to a DNA-binding domain, stimulates transcription. We demonstrate that a short region at the C-terminus of the FCP1 protein is required and sufficient for activation, indicating that neither the N-terminal phosphatase domain nor the BRCT domains are required for transcription activity of DNA-bound FCP1. In addition, we demonstrate that the C-terminus region of FCP1 suffices for efficient binding in vivo to the RAP74 subunit of TFIIF and is also required for the exclusive nuclear localization of the protein. These findings suggest a role for FCP1 as a positive regulator of RNAP II transcription." @default.
• W2006028144 created "2016-06-24" @default.
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• W2006028144 date "2001-09-01" @default.
• W2006028144 modified "2023-09-23" @default.
• W2006028144 title "Transcription activation by targeted recruitment of the RNA polymerase II CTD phosphatase FCP1" @default.
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• W2006028144 doi "https://doi.org/10.1093/nar/29.17.3539" @default.
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