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- W2006029105 abstract "Abstract A screening method was developed for detection of bacterial mutants having active enzymes with altered electrophoretic mobility. The method is based on the use of a mixture of several clones, and examination of an extract of the mixture electrophoretically. A variant enzyme will thus be detectable by its position apart from the mixture of wild-type enzymes. Following exposure to a mutagenic agent, five mutants of E. coli K12 were detected and isolated. Two of these have variant MDH (malate dehydrogenates), the others have variant forms of 6-phosphogluconate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, and esterase. Preliminary mapping of the MDH locus has been performed." @default.
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- W2006029105 date "1973-06-01" @default.
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- W2006029105 title "Isolation of induced mutations in E. coli affecting the electrophoretic mobility of enzymes" @default.
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- W2006029105 doi "https://doi.org/10.1016/0027-5107(73)90207-8" @default.
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