FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2006052043> ?p ?o ?g. }

• W2006052043 endingPage "111" @default.
• W2006052043 startingPage "104" @default.
• W2006052043 abstract "IL-31 is a novel cytokine that has been implicated in allergic diseases such as atopic dermatitis and more recently asthma. While IL-31 has been well studied in skin conditions such as atopic dermatitis, little is known about the role IL-31 plays in asthma and specifically the differentiation process of the bronchial epithelium, which is central to the pathogenesis of allergic asthma.We examined the effects of IL-13 (20 ng/ml), IL-31 (20 ng/ml) and an IL-13/IL-31 combination stimulation (20 ng/ml each) on the in vitro mucociliary differentiation of paediatric bronchial epithelial cells (PBECs) from healthy patients (n=6). IL-31 receptor (IL-31-RA) expression, markers of differentiation (goblet and ciliated cells), transepithelial electrical resistance (TEER), quantification of goblet and ciliated cells, real time PCR for MUC5AC, ELISA for VEGF, EGF and MCP-1 (CCL-2) and ELISA for MUC5AC were assessed.We found that well-differentiated PBECs expressed IL-31-RA however it's expression did not increase upon stimulation with IL-31 or either of the other treatments. TEER indicated good formation of tight junctions which was found to be similar across all treatment groups (p=0.9). We found that IL-13 alone significantly reduced the number of ciliated cells compared with unstimulated (IL-13 stimuation: mean=4.8% (SD=2.5); unstimulated: mean=15.9%, (SD=7.4), p<0.01). IL-31 stimulation alone had no effect on ciliated cells whereas the IL-13/IL-31 combination stimulation significantly reduced the number of ciliated cells compared with control (IL-13/IL-31 combination: mean=5.1% (SD=4.6); unstimulated: mean=15.9%, (SD=7.4), p<0.01). We did not find that the combination of IL-13 and IL-31 had any additional effects to that of IL-13 alone. MUC5AC mRNA and secreted mucin was found in similar levels between unstimulated and all treatments, however IL-13 increased levels of MUC5AC mRNA by a factor of 2.84, albeit not significantly, compared with unstimulated cultures (IL-13 stimulation: mean=2.84 (SD=3.79); unstimulated: mean=1.0).IL-31RA receptor is present on well-differentiated paediatric bronchial epithelial cells. IL-31 does not exhibit any detrimental effects on mucociliary differentiation. IL-31 does not appear to have a synergistic effect when combined in culture with IL-13, in the differentiation process." @default.
• W2006052043 created "2016-06-24" @default.
• W2006052043 creator A5011606845 @default.
• W2006052043 creator A5025523751 @default.
• W2006052043 creator A5037125586 @default.
• W2006052043 creator A5038193651 @default.
• W2006052043 creator A5040756420 @default.
• W2006052043 creator A5087865591 @default.
• W2006052043 date "2012-01-01" @default.
• W2006052043 modified "2023-09-26" @default.
• W2006052043 title "IL-31 does not induce normal human ciliated epithelial cells to differentiate into a phenotype consistent with the pathophysiology of asthma" @default.
• W2006052043 cites W1522844639 @default.
• W2006052043 cites W1733756185 @default.
• W2006052043 cites W1830548608 @default.
• W2006052043 cites W1975438745 @default.
• W2006052043 cites W1976114060 @default.
• W2006052043 cites W1988022838 @default.
• W2006052043 cites W1997452530 @default.
• W2006052043 cites W1998921059 @default.
• W2006052043 cites W2000228858 @default.
• W2006052043 cites W2017212450 @default.
• W2006052043 cites W2020199484 @default.
• W2006052043 cites W2024990391 @default.
• W2006052043 cites W2031501484 @default.
• W2006052043 cites W2032062642 @default.
• W2006052043 cites W2045121129 @default.
• W2006052043 cites W2059079830 @default.
• W2006052043 cites W2061821170 @default.
• W2006052043 cites W2065149104 @default.
• W2006052043 cites W2074715302 @default.
• W2006052043 cites W2087714421 @default.
• W2006052043 cites W2091825459 @default.
• W2006052043 cites W2092100225 @default.
• W2006052043 cites W2102975269 @default.
• W2006052043 cites W2107942850 @default.
• W2006052043 cites W2115531278 @default.
• W2006052043 cites W2120184224 @default.
• W2006052043 cites W2124127935 @default.
• W2006052043 cites W2142555112 @default.
• W2006052043 cites W2142559165 @default.
• W2006052043 cites W2147684493 @default.
• W2006052043 cites W2148312122 @default.
• W2006052043 cites W2165694323 @default.
• W2006052043 cites W2168675532 @default.
• W2006052043 cites W2171494815 @default.
• W2006052043 cites W2232581351 @default.
• W2006052043 cites W4237911065 @default.
• W2006052043 doi "https://doi.org/10.1016/j.rinim.2012.05.001" @default.
• W2006052043 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3862348" @default.
• W2006052043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24371573" @default.
• W2006052043 hasPublicationYear "2012" @default.
• W2006052043 type Work @default.
• W2006052043 sameAs 2006052043 @default.
• W2006052043 citedByCount "5" @default.
• W2006052043 countsByYear W20060520432016 @default.
• W2006052043 countsByYear W20060520432017 @default.
• W2006052043 countsByYear W20060520432018 @default.
• W2006052043 countsByYear W20060520432022 @default.
• W2006052043 crossrefType "journal-article" @default.
• W2006052043 hasAuthorship W2006052043A5011606845 @default.
• W2006052043 hasAuthorship W2006052043A5025523751 @default.
• W2006052043 hasAuthorship W2006052043A5037125586 @default.
• W2006052043 hasAuthorship W2006052043A5038193651 @default.
• W2006052043 hasAuthorship W2006052043A5040756420 @default.
• W2006052043 hasAuthorship W2006052043A5087865591 @default.
• W2006052043 hasBestOaLocation W20060520431 @default.
• W2006052043 hasConcept C10162356 @default.
• W2006052043 hasConcept C126322002 @default.
• W2006052043 hasConcept C134018914 @default.
• W2006052043 hasConcept C142724271 @default.
• W2006052043 hasConcept C170493617 @default.
• W2006052043 hasConcept C202751555 @default.
• W2006052043 hasConcept C203014093 @default.
• W2006052043 hasConcept C24998067 @default.
• W2006052043 hasConcept C2776042228 @default.
• W2006052043 hasConcept C2778329239 @default.
• W2006052043 hasConcept C2778690821 @default.
• W2006052043 hasConcept C2780942790 @default.
• W2006052043 hasConcept C32046619 @default.
• W2006052043 hasConcept C55493867 @default.
• W2006052043 hasConcept C71924100 @default.
• W2006052043 hasConcept C74172505 @default.
• W2006052043 hasConcept C86803240 @default.
• W2006052043 hasConceptScore W2006052043C10162356 @default.
• W2006052043 hasConceptScore W2006052043C126322002 @default.
• W2006052043 hasConceptScore W2006052043C134018914 @default.
• W2006052043 hasConceptScore W2006052043C142724271 @default.
• W2006052043 hasConceptScore W2006052043C170493617 @default.
• W2006052043 hasConceptScore W2006052043C202751555 @default.
• W2006052043 hasConceptScore W2006052043C203014093 @default.
• W2006052043 hasConceptScore W2006052043C24998067 @default.
• W2006052043 hasConceptScore W2006052043C2776042228 @default.
• W2006052043 hasConceptScore W2006052043C2778329239 @default.
• W2006052043 hasConceptScore W2006052043C2778690821 @default.
• W2006052043 hasConceptScore W2006052043C2780942790 @default.
• W2006052043 hasConceptScore W2006052043C32046619 @default.
• W2006052043 hasConceptScore W2006052043C55493867 @default.
• W2006052043 hasConceptScore W2006052043C71924100 @default.

• next