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• W2006084229 abstract "The purpose of this study was to investigate the generation of superoxide anion radical, O2-, by Kupffer cells in vivo in rat liver. Phorbol myristate acetate (PMA) was infused into perfused rat liver which had been continuously infused with 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo- [1,2-a]pyrazin-3-one (MCLA, a highly sensitive luminescence reagent for O2-) and the MCLA luminescence from the liver surface was detected with a sensitive photon counter. Under identical conditions, but without MCLA infusion, PMA and nitro blue tetrazolium were infused to verify generation of O2- by formation of formazan deposition. Based on MCLA luminescence and formazan deposition, both dependent on the O2- reaction, we have concluded that liver Kupffer cells generate O2- in vivo in response to stimuli such as PMA. Further, the generation of O2- by Kupffer cells may be by the same mechanism as displayed by macrophages." @default.
• W2006084229 created "2016-06-24" @default.
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• W2006084229 date "1993-12-06" @default.
• W2006084229 modified "2023-10-17" @default.
• W2006084229 title "The first application of a chemiluminescence probe, 2‐methyl‐6‐[<i>P</i>‐methoxyphenyl]‐3,7‐dihydroimidazo[1,2‐α]pyrazin‐3‐one (MCLA), for detecting O₂⁻ production, in vitro, from Kupffer cells stimulated by phorbol myristate acetate" @default.
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• W2006084229 doi "https://doi.org/10.1016/0014-5793(93)80722-7" @default.
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