Matches in SemOpenAlex for { <https://semopenalex.org/work/W2006087145> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2006087145 abstract "Oxygen regulates embryo development at both the cleavage and post compaction stages. In this study we investigated the effects of atmospheric oxygen during the preimplantation stages, on the post-compaction embryonic metabolome through quantification of glucose consumption and amino acid utilization. Zygotes obtained from F1 hybrid mice (C57BLxCBA) were randomly allocated to either 5 or 20% oxygen. In the first experiment, following the first 48 h of culture, embryos were cultured individually in 1 μL drops of modified G2 medium (0.5 mM glucose) and moved to fresh drops of medium every 24 h. The glucose concentration in the spent media samples, including controls containing no embryo, was determined by microfluorimetry. In the second experiment, embryos which had developed to the early blastocyst stage after 72 h were cultured for a further 24 h in groups of 10 in 2 μL drops of G2. Analysis of amino acid utilization was performed using liquid chromatography-triple quadrupole mass spectrometry. Glucose consumption by embryos cultured in 5% oxygen was significantly greater on day 4 and day 5 (4.89 ± 0.29 and 6.13 ± 0.41 pmol/embryo/h) compared to embryos cultured in 20% oxygen (2.59 ± 0.40 and 5.09 ± 0.28 pmol/embryo/h; P < 0.05). In contrast amino acid utilisation by embryos cultured in 5% oxygen was significantly less than embryos cultured in 20% oxygen (P < 0.05). The data generated will help to determine the aetiology of oxygen toxicity to the preimplantation embryo. Higher glucose utilisation by embryos in 5% oxygen is consistent with their improved development. Conversely, the increased utilisation of amino acids by blastocysts in 20% oxygen may reflect an adaptation to increased oxidative stress as a result of culture in a non-physiological oxygen concentration. This study demonstrates that atmospheric oxygen during the preimplantation period perturbs the embryonic metabolome which results in a compensatory increase in amino acid utilisation." @default.
- W2006087145 created "2016-06-24" @default.
- W2006087145 creator A5064026512 @default.
- W2006087145 creator A5081557829 @default.
- W2006087145 date "2010-01-01" @default.
- W2006087145 modified "2023-09-26" @default.
- W2006087145 title "174. ATMOSPHERIC OXYGEN ALTERS THE EMBRYONIC METABOLOME AS QUANTIFIED BY CARBOHYDRATE UPTAKE AND AMINO ACID UTILISATION" @default.
- W2006087145 doi "https://doi.org/10.1071/srb10abs174" @default.
- W2006087145 hasPublicationYear "2010" @default.
- W2006087145 type Work @default.
- W2006087145 sameAs 2006087145 @default.
- W2006087145 citedByCount "0" @default.
- W2006087145 crossrefType "journal-article" @default.
- W2006087145 hasAuthorship W2006087145A5064026512 @default.
- W2006087145 hasAuthorship W2006087145A5081557829 @default.
- W2006087145 hasConcept C135870905 @default.
- W2006087145 hasConcept C16685009 @default.
- W2006087145 hasConcept C178790620 @default.
- W2006087145 hasConcept C185592680 @default.
- W2006087145 hasConcept C196843134 @default.
- W2006087145 hasConcept C2777477808 @default.
- W2006087145 hasConcept C2778177303 @default.
- W2006087145 hasConcept C2779335785 @default.
- W2006087145 hasConcept C515207424 @default.
- W2006087145 hasConcept C540031477 @default.
- W2006087145 hasConcept C55493867 @default.
- W2006087145 hasConcept C71924100 @default.
- W2006087145 hasConcept C86803240 @default.
- W2006087145 hasConcept C87073359 @default.
- W2006087145 hasConcept C89169090 @default.
- W2006087145 hasConcept C95444343 @default.
- W2006087145 hasConceptScore W2006087145C135870905 @default.
- W2006087145 hasConceptScore W2006087145C16685009 @default.
- W2006087145 hasConceptScore W2006087145C178790620 @default.
- W2006087145 hasConceptScore W2006087145C185592680 @default.
- W2006087145 hasConceptScore W2006087145C196843134 @default.
- W2006087145 hasConceptScore W2006087145C2777477808 @default.
- W2006087145 hasConceptScore W2006087145C2778177303 @default.
- W2006087145 hasConceptScore W2006087145C2779335785 @default.
- W2006087145 hasConceptScore W2006087145C515207424 @default.
- W2006087145 hasConceptScore W2006087145C540031477 @default.
- W2006087145 hasConceptScore W2006087145C55493867 @default.
- W2006087145 hasConceptScore W2006087145C71924100 @default.
- W2006087145 hasConceptScore W2006087145C86803240 @default.
- W2006087145 hasConceptScore W2006087145C87073359 @default.
- W2006087145 hasConceptScore W2006087145C89169090 @default.
- W2006087145 hasConceptScore W2006087145C95444343 @default.
- W2006087145 hasLocation W20060871451 @default.
- W2006087145 hasOpenAccess W2006087145 @default.
- W2006087145 hasPrimaryLocation W20060871451 @default.
- W2006087145 hasRelatedWork W1518384220 @default.
- W2006087145 hasRelatedWork W1526991110 @default.
- W2006087145 hasRelatedWork W1591186997 @default.
- W2006087145 hasRelatedWork W1674049381 @default.
- W2006087145 hasRelatedWork W1852831759 @default.
- W2006087145 hasRelatedWork W2017501141 @default.
- W2006087145 hasRelatedWork W2026336054 @default.
- W2006087145 hasRelatedWork W2030187385 @default.
- W2006087145 hasRelatedWork W2033957722 @default.
- W2006087145 hasRelatedWork W2039228769 @default.
- W2006087145 hasRelatedWork W2068985425 @default.
- W2006087145 hasRelatedWork W2091263173 @default.
- W2006087145 hasRelatedWork W2145676352 @default.
- W2006087145 hasRelatedWork W2153206937 @default.
- W2006087145 hasRelatedWork W2164666014 @default.
- W2006087145 hasRelatedWork W2230592817 @default.
- W2006087145 hasRelatedWork W2559090516 @default.
- W2006087145 hasRelatedWork W2559471385 @default.
- W2006087145 hasRelatedWork W2588322681 @default.
- W2006087145 hasRelatedWork W1970257116 @default.
- W2006087145 isParatext "false" @default.
- W2006087145 isRetracted "false" @default.
- W2006087145 magId "2006087145" @default.
- W2006087145 workType "article" @default.