FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2006087311> ?p ?o ?g. }

• W2006087311 endingPage "128" @default.
• W2006087311 startingPage "115" @default.
• W2006087311 abstract "IL-10 is a potent mediator of human B cell growth and plasma cell formation However, signal transduction of IL-10 in B cells is poorly understood. In this study the effect of IL-10 on the expression of the protooncogene c-fos was investigated, because Fos plays a potential role in the regulation of B cell proliferation and differentiation. B cells were purified from buffy coat preparations of healthy blood donors by positive selection using an anti CD20 monoclonal antibody and a MiniMACS separation unit. B cells were prestimulated with SAC for 48 hrs Then, cells were incubated with medium or IL-10 (100 ng/ml) for 10 to 120 min. RNA was extracted by phenol/chloroform and c-fos expression was analyzed by PCR assisted mRNA assay. A significant 2–4 fold increase of c-fos expression was observed within 30 min of stimulation with IL-10 (p < 0,01). After 2 hrs c-fos expression declined to basal levels The effect of IL-10 was dose-dependent with a maximum stimulation using 100 ng/ml of IL-10. The IL-10 effect on c-fos expression was not blocked by polymyxin B. Using the tyrosine kinase inhibitor genistein (10μM) a complete inhibition of IL-10 induced c-fos expression was observed In addition, H-7 (10μM), a specific inhibitor of serine/threonin kinases, significantly blocked IL-10 mediated c-fos expression (p < 0,05). In conclusion, these data show that IL-10 induces c-fos expression in human B-cells by activation of tyrosine and serine/threonin kinases. Since this is the first report on IL-10 induced signal transduction, these data may help to identify the intracellular mechanisms by which IL-10 stimulates human B-cells." @default.
• W2006087311 created "2016-06-24" @default.
• W2006087311 creator A5001375613 @default.
• W2006087311 creator A5012946031 @default.
• W2006087311 creator A5021490889 @default.
• W2006087311 creator A5042473693 @default.
• W2006087311 creator A5051979326 @default.
• W2006087311 date "1996-01-01" @default.
• W2006087311 modified "2023-09-26" @default.
• W2006087311 title "Interleukin 10 Induced C-FOS Expression in Human B Cells by Activation of Divergent Protein Kinases" @default.
• W2006087311 cites W1970781119 @default.
• W2006087311 cites W1985902728 @default.
• W2006087311 cites W1987897953 @default.
• W2006087311 cites W2009754477 @default.
• W2006087311 cites W2019392447 @default.
• W2006087311 cites W2036072927 @default.
• W2006087311 cites W2058744360 @default.
• W2006087311 cites W2084990572 @default.
• W2006087311 cites W2139573199 @default.
• W2006087311 cites W2148586485 @default.
• W2006087311 cites W2161347217 @default.
• W2006087311 doi "https://doi.org/10.3109/08820139609059296" @default.
• W2006087311 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8675228" @default.
• W2006087311 hasPublicationYear "1996" @default.
• W2006087311 type Work @default.
• W2006087311 sameAs 2006087311 @default.
• W2006087311 citedByCount "10" @default.
• W2006087311 crossrefType "journal-article" @default.
• W2006087311 hasAuthorship W2006087311A5001375613 @default.
• W2006087311 hasAuthorship W2006087311A5012946031 @default.
• W2006087311 hasAuthorship W2006087311A5021490889 @default.
• W2006087311 hasAuthorship W2006087311A5042473693 @default.
• W2006087311 hasAuthorship W2006087311A5051979326 @default.
• W2006087311 hasConcept C104317684 @default.
• W2006087311 hasConcept C134018914 @default.
• W2006087311 hasConcept C150194340 @default.
• W2006087311 hasConcept C153911025 @default.
• W2006087311 hasConcept C184235292 @default.
• W2006087311 hasConcept C185592680 @default.
• W2006087311 hasConcept C24998067 @default.
• W2006087311 hasConcept C2780323455 @default.
• W2006087311 hasConcept C55493867 @default.
• W2006087311 hasConcept C62478195 @default.
• W2006087311 hasConcept C86803240 @default.
• W2006087311 hasConceptScore W2006087311C104317684 @default.
• W2006087311 hasConceptScore W2006087311C134018914 @default.
• W2006087311 hasConceptScore W2006087311C150194340 @default.
• W2006087311 hasConceptScore W2006087311C153911025 @default.
• W2006087311 hasConceptScore W2006087311C184235292 @default.
• W2006087311 hasConceptScore W2006087311C185592680 @default.
• W2006087311 hasConceptScore W2006087311C24998067 @default.
• W2006087311 hasConceptScore W2006087311C2780323455 @default.
• W2006087311 hasConceptScore W2006087311C55493867 @default.
• W2006087311 hasConceptScore W2006087311C62478195 @default.
• W2006087311 hasConceptScore W2006087311C86803240 @default.
• W2006087311 hasIssue "1-2" @default.
• W2006087311 hasLocation W20060873111 @default.
• W2006087311 hasLocation W20060873112 @default.
• W2006087311 hasOpenAccess W2006087311 @default.
• W2006087311 hasPrimaryLocation W20060873111 @default.
• W2006087311 hasRelatedWork W1995478390 @default.
• W2006087311 hasRelatedWork W2009966535 @default.
• W2006087311 hasRelatedWork W2060267645 @default.
• W2006087311 hasRelatedWork W2067792075 @default.
• W2006087311 hasRelatedWork W2085668533 @default.
• W2006087311 hasRelatedWork W2161361144 @default.
• W2006087311 hasRelatedWork W2350911639 @default.
• W2006087311 hasRelatedWork W2364940155 @default.
• W2006087311 hasRelatedWork W2387489392 @default.
• W2006087311 hasRelatedWork W2436690931 @default.
• W2006087311 hasVolume "25" @default.
• W2006087311 isParatext "false" @default.
• W2006087311 isRetracted "false" @default.
• W2006087311 magId "2006087311" @default.
• W2006087311 workType "article" @default.