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- W2006087781 abstract "The group of biologically important amines includes putrescine, spermidine and spermine, as well as agmatine, which is a guanidino-amine. There is considerable evidence supporting a role of these amines in the etiology and pathology of mental disorders. We have previously developed a quantitative GC-MS method for simultaneous measurement of three major polyamines to support our studies linking polyamines to mental disorders. However, a unique GC-MS method is required for agmatine. To efficiently extract agmatine from postmortem brain tissues, we developed an isopropanol based liquid–liquid extraction protocol using potassium carbonate as a salting-out agent which showed a much greater recovery than n-butanol used in earlier methods. The GC-MS analysis employed hexafluoroacetylacetone as derivatization reagent and was carried out using negative chemical ionization with total ion and selected ion monitoring. 15N4-Agmatine was synthesized from 15N4-L-arginine and used as internal standard in a conventional stable isotope dilution assay. This method accurately measures the level of agmatine from very small quantities (10–20 mg) of postmortem brain tissue, with a quantitation limit down to 1 ng/g of wet tissue. The limit of detection is 0.01 ng/g of wet tissue. Copyright © 2010 John Wiley & Sons, Ltd." @default.
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- W2006087781 date "2010-04-13" @default.
- W2006087781 modified "2023-10-17" @default.
- W2006087781 title "A novel liquid-liquid extraction and stable isotope dilution NCI-GC-MS method for quantitation of agmatine in postmortem brain cortex" @default.
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- W2006087781 doi "https://doi.org/10.1002/jms.1742" @default.
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