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- W2006157027 abstract "To deliver brain-derived neurotrophic factor (BDNF) to the central nervous system, we sought to attain adenovirus-mediated transfer and expression of the gene in both in vitro and in vivo experiments. For this purpose, we constructed AxCA-BDNF, a recombinant adenoviral vector containing the BDNF cDNA expression cassette. Reverse transcription polymerase chain reaction analyses of the infected HeLa cells and the transduced mouse brain revealed successful expression of the BDNF gene both in vitro and in vivo. The results of a survival assay of chick dorsal root ganglion cells showed that the produced BDNF was biologically active. We consider, therefore, this newly constructed recombinant adenovirus to be a useful tool to deliver BDNF to degenerating neurons and to be applicable to gene therapy Of neurodegenerative diseases and nerve trauma." @default.
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- W2006157027 date "1995-07-01" @default.
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- W2006157027 title "Construction and Characterization of Adenoviral Vector Expressing Biologically-Active Brain-Derived Neurotrophic Factor" @default.
- W2006157027 doi "https://doi.org/10.1006/bbrc.1995.2027" @default.
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