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- W2006185252 abstract "Freeze-drying and glycolmethacrylate embedment (FDGE), without prior fixation and chemical dehydration, has proven useful in the study of the progression of lesions associated with diethylnitrosamine-induced liver neoplasia in medaka (Oryzias latipes). Localization of specific enzymes including acid phosphatase (ACP), alkaline phosphatase (ALKP), gamma-glutamyl transpeptidase (GGT), uridine diphosphate glucose dehydrogenase (UDPGDH), quinone oxidoreductase or DT diaphorase (DTD), and glucose-6-phosphate dehydrogenase (G6PDH) was achieved histochemically and correlated, in serial sections, with the conventional histologic stain, hematoxylin and eosin. Phenotypes of enzyme-altered foci and subsequent lesions in the promotion and progression of hepatocarcinogenesis were determined. GGT and G6PDH marked toxic and inflammatory phases respectively, while GGT, DTD, ALKP, ACP, G6PDH and UDPGDH proved useful in differentiating phenotypically altered hepatocytes during the later stages of neoplastic development. FDGE permitted the evaluation of multiple tissue markers in serial sections. limited the development of diffusion artifacts, produced higher resolution than cryostat sections and proved nearly as good as conventional light microscopy for routine survey. Integration of morphologic and biochemical data within microscopic lesions in a single small liver has been achieved." @default.
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- W2006185252 date "1993-02-01" @default.
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- W2006185252 title "Detection of enzyme histochemical markers of hepatic preneoplasia and neoplasia in medaka (Oryzias latipes)" @default.
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- W2006185252 doi "https://doi.org/10.1016/0166-445x(93)90069-d" @default.
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