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• W2006196154 abstract "Current 13C-metabolic flux analysis methods were reviewed as well as the weakness of the conventional metabolic flux analysis without 13C-labeled experiments. Although it has been recognized that 13C-labeling technique is powerful in estimating the metabolic fluxes, and the program-based flux analysis is necessary, one may not be confident with the result obtained without experiences and exhaustive trial and errors in practice due to its black box nature. In the present article, we call attention to the importance of investigating the relationships between fluxes and isotopomer or mass isotopomer distributions to understand the mechanism of generating specific isotopomers. Then, the experimental design for the preferred mixture of the specific 13C-labeled substrate was discussed. The effect of the reversibility in the bidirectional flux on the isotopomer distribution was also mentioned, and it was shown why the reliability of the bidirectional fluxes becomes lower. Moreover, by noting that recent development of measurement techniques enables us to measure the isotopomer patterns of intracellular metabolites instead of proteinogenic amino acids, it is mentioned that this enables us to estimate the flux changes during time-variant batch culture. Some future perspectives are discussed in relation to the integration of different levels of information in the cell." @default.
• W2006196154 created "2016-06-24" @default.
• W2006196154 creator A5036678316 @default.
• W2006196154 creator A5067288478 @default.
• W2006196154 date "2010-12-01" @default.
• W2006196154 modified "2023-10-16" @default.
• W2006196154 title "Current status of 13C-metabolic flux analysis and future perspectives" @default.
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