FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2006205853> ?p ?o ?g. }

• W2006205853 endingPage "126" @default.
• W2006205853 startingPage "118" @default.
• W2006205853 abstract "The Smcp mRNA encoding the sperm mitochondria-associated cysteine-rich protein is translationally repressed in round spermatids and translationally active in elongated spermatids. The patterns of transcription and translation of fusions of the Smcp promoter, the green fluorescent protein coding region (Gfp) and various combination of the Smcp and Gfp 5′ UTR and 3′ UTR have been studied in transgenic mice. 518 nt of Smcp 5′ flanking region and 8 nt of 5′ UTR drive transcription of mRNAs containing the Gfp coding region in early round spermatids at the same transcription start site as the natural Smcp gene. Transcripts containing both the Gfp 5′ and 3′ UTRs are translationally active in step 2 spermatids as detected by GFP fluorescence in squashes of living seminiferous tubules from adult testes, and the presence of polysomal mRNAs in sucrose gradient analyses of testes from 21-day-old prepubertal mice, which contain early round spermatids and lack elongated spermatids. By comparison, expression of GFP is delayed until steps 5 and 10, respectively, in transcripts containing the Smcp 5′ UTR and Gfp 3′ UTR and the Gfp 5′ UTR and the Smcp 3′ UTR. Sucrose gradient analysis of 21-day-old testes demonstrates that transcripts containing the Smcp 3′ UTR exhibit a bimodal distribution in free-mRNPs and polysomes, indicating that the 3′ UTR blocks the expression of GFP after the transcripts have entered the elongation phase, a mechanism that may involve microRNAs. The Smcp 5′ UTR reduces the levels and size of polysomes in adult testis. In addition, the natural Smcp mRNA contains a positive control element that counteracts the inhibition of translation by the Smcp 5′ UTR in adult testis, and the Smcp 3′ UTR strongly localizes GFP fluorescence in step 10 spermatids." @default.
• W2006205853 created "2016-06-24" @default.
• W2006205853 creator A5041490529 @default.
• W2006205853 creator A5043189146 @default.
• W2006205853 creator A5062431352 @default.
• W2006205853 date "2006-09-01" @default.
• W2006205853 modified "2023-10-16" @default.
• W2006205853 title "The 5′ UTR and 3′ UTR of the sperm mitochondria-associated cysteine-rich protein mRNA regulate translation in spermatids by multiple mechanisms in transgenic mice" @default.
• W2006205853 cites W1488566500 @default.
• W2006205853 cites W1528915146 @default.
• W2006205853 cites W1558737283 @default.
• W2006205853 cites W1568945461 @default.
• W2006205853 cites W1980084095 @default.
• W2006205853 cites W1984672754 @default.
• W2006205853 cites W1986925875 @default.
• W2006205853 cites W1991136563 @default.
• W2006205853 cites W2003371280 @default.
• W2006205853 cites W2012999170 @default.
• W2006205853 cites W2015015700 @default.
• W2006205853 cites W2028267599 @default.
• W2006205853 cites W2032945651 @default.
• W2006205853 cites W2037437212 @default.
• W2006205853 cites W2055148233 @default.
• W2006205853 cites W2071709406 @default.
• W2006205853 cites W2072153827 @default.
• W2006205853 cites W2076289080 @default.
• W2006205853 cites W2084489095 @default.
• W2006205853 cites W2084514870 @default.
• W2006205853 cites W2094604037 @default.
• W2006205853 cites W2109535551 @default.
• W2006205853 cites W2110341505 @default.
• W2006205853 cites W2113083989 @default.
• W2006205853 cites W2116077211 @default.
• W2006205853 cites W2118854491 @default.
• W2006205853 cites W2119836880 @default.
• W2006205853 cites W2124666089 @default.
• W2006205853 cites W2127651312 @default.
• W2006205853 cites W2145155005 @default.
• W2006205853 cites W2146138309 @default.
• W2006205853 cites W2154917812 @default.
• W2006205853 cites W2166840544 @default.
• W2006205853 cites W2167418617 @default.
• W2006205853 cites W2167668982 @default.
• W2006205853 cites W2270487047 @default.
• W2006205853 cites W4243584423 @default.
• W2006205853 doi "https://doi.org/10.1016/j.ydbio.2006.04.468" @default.
• W2006205853 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16759650" @default.
• W2006205853 hasPublicationYear "2006" @default.
• W2006205853 type Work @default.
• W2006205853 sameAs 2006205853 @default.
• W2006205853 citedByCount "17" @default.
• W2006205853 countsByYear W20062058532013 @default.
• W2006205853 countsByYear W20062058532014 @default.
• W2006205853 countsByYear W20062058532015 @default.
• W2006205853 countsByYear W20062058532016 @default.
• W2006205853 countsByYear W20062058532018 @default.
• W2006205853 countsByYear W20062058532019 @default.
• W2006205853 crossrefType "journal-article" @default.
• W2006205853 hasAuthorship W2006205853A5041490529 @default.
• W2006205853 hasAuthorship W2006205853A5043189146 @default.
• W2006205853 hasAuthorship W2006205853A5062431352 @default.
• W2006205853 hasConcept C102230213 @default.
• W2006205853 hasConcept C104317684 @default.
• W2006205853 hasConcept C105580179 @default.
• W2006205853 hasConcept C12464233 @default.
• W2006205853 hasConcept C138885662 @default.
• W2006205853 hasConcept C142613039 @default.
• W2006205853 hasConcept C149364088 @default.
• W2006205853 hasConcept C153911025 @default.
• W2006205853 hasConcept C179926584 @default.
• W2006205853 hasConcept C22667442 @default.
• W2006205853 hasConcept C41895202 @default.
• W2006205853 hasConcept C54355233 @default.
• W2006205853 hasConcept C65666806 @default.
• W2006205853 hasConcept C67705224 @default.
• W2006205853 hasConcept C86803240 @default.
• W2006205853 hasConcept C88478588 @default.
• W2006205853 hasConcept C89604277 @default.
• W2006205853 hasConcept C95444343 @default.
• W2006205853 hasConceptScore W2006205853C102230213 @default.
• W2006205853 hasConceptScore W2006205853C104317684 @default.
• W2006205853 hasConceptScore W2006205853C105580179 @default.
• W2006205853 hasConceptScore W2006205853C12464233 @default.
• W2006205853 hasConceptScore W2006205853C138885662 @default.
• W2006205853 hasConceptScore W2006205853C142613039 @default.
• W2006205853 hasConceptScore W2006205853C149364088 @default.
• W2006205853 hasConceptScore W2006205853C153911025 @default.
• W2006205853 hasConceptScore W2006205853C179926584 @default.
• W2006205853 hasConceptScore W2006205853C22667442 @default.
• W2006205853 hasConceptScore W2006205853C41895202 @default.
• W2006205853 hasConceptScore W2006205853C54355233 @default.
• W2006205853 hasConceptScore W2006205853C65666806 @default.
• W2006205853 hasConceptScore W2006205853C67705224 @default.
• W2006205853 hasConceptScore W2006205853C86803240 @default.
• W2006205853 hasConceptScore W2006205853C88478588 @default.
• W2006205853 hasConceptScore W2006205853C89604277 @default.
• W2006205853 hasConceptScore W2006205853C95444343 @default.
• W2006205853 hasIssue "1" @default.

• next