Matches in SemOpenAlex for { <https://semopenalex.org/work/W2006207612> ?p ?o ?g. }
- W2006207612 endingPage "934" @default.
- W2006207612 startingPage "929" @default.
- W2006207612 abstract "One of the early events in the pathway of receptor-mediated endocytosis is the acidification of the newly formed endocytic vesicle. To examine the mechanism of acidification, we used fluorescein-labeled alpha 2-macroglobulin (F-alpha 2M) as a probe for endocytic vesicle pH. Changes in pH were determined from the change in fluorescein fluorescence at 490-nm excitation as measured with a microscope spectrofluorometer. After endocytosis of F-alpha 2M, mouse fibroblast cells were permeabilized by brief exposure to the detergent digitonin. Treatment with the ionophore monensin or the protonophore carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) caused a rapid increase in the pH of the endocytic vesicle. Upon removal of the ionophore, the endocytic vesicle rapidly acidified only when MgATP or MgGTP was added. Neither ADP nor the nonhydrolyzable analog, adenosine 5'-(beta, gamma-imido)triphosphate (AMP-PNP) could support acidification. The ATP-dependent acidification did not require a specific cation or anion in the external media. Acidification was insensitive to vanadate and amiloride but was inhibited by Zn2+ and the anion transport inhibitor diisothiocyanostilbene disulfonic acid (DIDS). We also examined the acidification of lysosomes with the permeabilized cell system, using fluorescein isothiocyanate dextran as probe. DIDS inhibited the ATP-dependent reacidification of lysosomes, although at a lower concentration than that for inhibition of endocytic vesicle reacidification. These results demonstrate that endocytic vesicles contain an ATP-dependent acidification mechanism that shares similar characteristics with the previously described lysosomal proton pump." @default.
- W2006207612 created "2016-06-24" @default.
- W2006207612 creator A5031841410 @default.
- W2006207612 creator A5053648650 @default.
- W2006207612 creator A5082887523 @default.
- W2006207612 date "1983-09-01" @default.
- W2006207612 modified "2023-10-10" @default.
- W2006207612 title "Acidification of endocytic vesicles by an ATP-dependent proton pump." @default.
- W2006207612 cites W1493832020 @default.
- W2006207612 cites W1500181036 @default.
- W2006207612 cites W1506188765 @default.
- W2006207612 cites W1514725762 @default.
- W2006207612 cites W1542880421 @default.
- W2006207612 cites W1551647770 @default.
- W2006207612 cites W1553513611 @default.
- W2006207612 cites W1558208511 @default.
- W2006207612 cites W156235783 @default.
- W2006207612 cites W1562698404 @default.
- W2006207612 cites W1570821412 @default.
- W2006207612 cites W1583400983 @default.
- W2006207612 cites W1608191411 @default.
- W2006207612 cites W167100554 @default.
- W2006207612 cites W1824817217 @default.
- W2006207612 cites W1963600085 @default.
- W2006207612 cites W1965509347 @default.
- W2006207612 cites W1982074683 @default.
- W2006207612 cites W1982379148 @default.
- W2006207612 cites W1986204805 @default.
- W2006207612 cites W1986529810 @default.
- W2006207612 cites W1990244025 @default.
- W2006207612 cites W1991630769 @default.
- W2006207612 cites W1992900472 @default.
- W2006207612 cites W1999795482 @default.
- W2006207612 cites W2001197606 @default.
- W2006207612 cites W2005910022 @default.
- W2006207612 cites W2020072455 @default.
- W2006207612 cites W2032836678 @default.
- W2006207612 cites W2035277465 @default.
- W2006207612 cites W2036127596 @default.
- W2006207612 cites W2043580386 @default.
- W2006207612 cites W2062545915 @default.
- W2006207612 cites W2064625798 @default.
- W2006207612 cites W2066663508 @default.
- W2006207612 cites W2093884254 @default.
- W2006207612 cites W2099826607 @default.
- W2006207612 cites W2110408216 @default.
- W2006207612 cites W2112055017 @default.
- W2006207612 cites W2125648721 @default.
- W2006207612 cites W2147347701 @default.
- W2006207612 cites W2149817638 @default.
- W2006207612 cites W2161106503 @default.
- W2006207612 cites W2164227469 @default.
- W2006207612 cites W2167948410 @default.
- W2006207612 cites W2409171504 @default.
- W2006207612 cites W73313321 @default.
- W2006207612 doi "https://doi.org/10.1083/jcb.97.3.929" @default.
- W2006207612 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2112557" @default.
- W2006207612 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6224803" @default.
- W2006207612 hasPublicationYear "1983" @default.
- W2006207612 type Work @default.
- W2006207612 sameAs 2006207612 @default.
- W2006207612 citedByCount "209" @default.
- W2006207612 countsByYear W20062076122012 @default.
- W2006207612 countsByYear W20062076122013 @default.
- W2006207612 countsByYear W20062076122014 @default.
- W2006207612 countsByYear W20062076122016 @default.
- W2006207612 countsByYear W20062076122017 @default.
- W2006207612 countsByYear W20062076122018 @default.
- W2006207612 countsByYear W20062076122019 @default.
- W2006207612 countsByYear W20062076122020 @default.
- W2006207612 countsByYear W20062076122021 @default.
- W2006207612 countsByYear W20062076122022 @default.
- W2006207612 countsByYear W20062076122023 @default.
- W2006207612 crossrefType "journal-article" @default.
- W2006207612 hasAuthorship W2006207612A5031841410 @default.
- W2006207612 hasAuthorship W2006207612A5053648650 @default.
- W2006207612 hasAuthorship W2006207612A5082887523 @default.
- W2006207612 hasBestOaLocation W20062076121 @default.
- W2006207612 hasConcept C12554922 @default.
- W2006207612 hasConcept C130316041 @default.
- W2006207612 hasConcept C134897140 @default.
- W2006207612 hasConcept C170493617 @default.
- W2006207612 hasConcept C181199279 @default.
- W2006207612 hasConcept C23265538 @default.
- W2006207612 hasConcept C2777330628 @default.
- W2006207612 hasConcept C2778920010 @default.
- W2006207612 hasConcept C2779014961 @default.
- W2006207612 hasConcept C2779134199 @default.
- W2006207612 hasConcept C2779533339 @default.
- W2006207612 hasConcept C2780224938 @default.
- W2006207612 hasConcept C28005876 @default.
- W2006207612 hasConcept C41625074 @default.
- W2006207612 hasConcept C55493867 @default.
- W2006207612 hasConcept C79747257 @default.
- W2006207612 hasConcept C79879829 @default.
- W2006207612 hasConcept C86803240 @default.
- W2006207612 hasConcept C95444343 @default.
- W2006207612 hasConceptScore W2006207612C12554922 @default.