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• W2006263845 abstract "The activities of many mammalian membrane proteins including G-protein coupled receptors are cholesterol-dependent. Unlike higher eukaryotes, yeast do not make cholesterol. Rather they make a related molecule called ergosterol. As cholesterol and ergosterol are biologically non-equivalent, the potential of yeast as hosts for overproducing mammalian membrane proteins has never been fully realised. To address this problem, we are trying to engineer a novel strain of Saccharomyces cerevisiae in which the cholesterol biosynthetic pathway of mammalian cells has been fully reconstituted. Thus far, we have created a modified strain that makes cholesterol-like sterols which has an increased capacity to make G-protein coupled receptors compared to control yeast." @default.
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• W2006263845 date "2011-12-01" @default.
• W2006263845 modified "2023-09-27" @default.
• W2006263845 title "GPCR production in a novel yeast strain that makes cholesterol-like sterols" @default.
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• W2006263845 doi "https://doi.org/10.1016/j.ymeth.2011.09.023" @default.
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