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- W2006292068 abstract "S449 Nitricoxide (NO) is an important intra and intercellular messenger in the nervous system. Recently, it has been reported that NO negatively modulates native GABAA receptors of rat brain. [1] GABAA receptor is considered to be an important site of action of anesthetics and sedatives [2] but there are only a few reports on NO and GABAA receptor. The present work was undertaken to clarify the site of GABAA receptor involved in the action of NO using recombinant GABA (A) receptors. Methods: mRNA transcribed in vitro from cDNA of mouse GABAA receptor subunit alpha1, beta2 and gamma2 s were expressed by injection into Xenopus oocytes. NOC-18 (2,2[prime]-hydroxynitrosohydrazono-bis-ethanamine) which releases NO independent of any metabolic pathways and releases no physiologically active by-products was used as a NO donor. The effects of NO on GABA induced C1 current in the oocytes coexpressing either alpha1 beta2 gamma2 s or alpha (1) beta2 subunits were recorded by two electrode voltage clamp method. To confirm the effect was solely due to NO, the NO extinguisher caboxy -PTIO was subsequently applied with NOC-18 and the disappearance of the effect was confirmed. Results: In alpha1 beta2 gamma2 s subunit composition, NOC-18 reduced GABA induced C1 current in dose dependent manner. In alpha1 beta (2) subunit composition, there was no significant change in the C1 current at 0.1 and 1mM of NOC-18 but at 10mM NOC-18, the C1 current significantly increased (p<0.05). Conclusions: Our results show that gamma2 s subunit is involved in the negative modulatory effect of NO. This modulatory effect may be the direct action of NO on the receptor. (Figure 1)Figure 1" @default.
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- W2006292068 date "1998-02-01" @default.
- W2006292068 modified "2023-10-14" @default.
- W2006292068 title "SUBUNIT DEPENDENT EFFECTS OF NITRIC OXIDE ON RECOMBINANT GABAA RECEPTOR EXPRESSED IN XENOPUS OOCYTES" @default.
- W2006292068 doi "https://doi.org/10.1097/00000539-199802001-00447" @default.
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