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- W2006298076 abstract "A dioxygenase from the edible basidiomycete Pleurotus sapidus, originally researched because of its distinct ability to convert the sequiterpene (+)-valencene to the valuable grapefruit aroma (+)-nootkatone, was identified as a potent lipoxygenase (LOXPsa1). Kinetic parameters, pH and temperature optima of the pure recombinant enzyme were determined using linoleic acid as the substrate. Km, vmax, and kcat were 40.3 μM, 130.3 U mg−1, and 157 s−1, respectively. The maximal enzymatic activity was found at pH 7.0 and 35 °C. Showing high specificity toward free linoleic acid, the enzyme was classified as lipoxygenase type 1. Conversion of linoleic acid yielded mainly (S)-13-hydroperoxy-9Z,11E-octadecadienoic acid (94% ee), as was confirmed by chiral HPLC analysis of the hydroperoxides. The amino acid sequence showed homology to lipoxygenases catalyzing S stereospecific oxygenation, and thus the enzyme was characterized as a 13S-lipoxygenase. This is the first lipoxygenase described to accept terpene hydrocarbons as substrates." @default.
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- W2006298076 date "2013-03-01" @default.
- W2006298076 modified "2023-10-18" @default.
- W2006298076 title "LOXPsa1, the first recombinant lipoxygenase from a basidiomycete fungus" @default.
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- W2006298076 doi "https://doi.org/10.1016/j.molcatb.2012.11.004" @default.
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