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- W2006301901 abstract "4-methylthio-3-butenyl isothiocyanate (4-MTBI) is released from 4-methylthio-3-butenyl glucosinolate (4-MTBG) in radish roots by endogenous myrosinase hydrolysis. In this research, factors influencing the extraction of 4-MTBI from radish roots by an endogenous myrosinase method, such as the time for endogenous myrosinase hydrolysis under 37 °C, the storage time after hydrolysis, were optimized. A new GC method was developed to determine 4-MTBI in radish roots, which was identified by GC-MS and compared with the authentic 4-MTBI. Through optimization of the starting point and increasement of column temperature, both the internal standard allyl isothiocyanate (AITC) and 4-MTBI were clearly separated with a capillary gas chromatography column HP-5. Coefficient of variation (CV) of GC analysis was 3.883%. Recovery percentage of AITC remained 90.3–105.4%. CV of determination and recovery percentage of sinigrin were 5.35% and 91.3–101.9%, respectively." @default.
- W2006301901 created "2016-06-24" @default.
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- W2006301901 date "2015-03-01" @default.
- W2006301901 modified "2023-10-18" @default.
- W2006301901 title "Extraction and determination of 4-methylthio-3-butenyl isothiocyanate in Chinese radish (Raphanus sativus L.) roots" @default.
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- W2006301901 doi "https://doi.org/10.1016/j.lwt.2014.10.014" @default.
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