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• W2006312193 abstract "We report for the first time the development of a sensitive and selective glucose biosensor based on the self-assembling of multiwall carbon nanotubes (MWCNTs) dispersed in polyhistidine (Polyhis) and glucose oxidase (GOx) on glassy carbon electrodes (GCE). The supramolecular architecture was characterized by SEM, FT-IR and electrochemical techniques. The optimum multistructure was obtained with five (MWCNT-Polyhis/GOx) bilayers and one layer of Nafion as anti-interferent barrier. The sensitivity at 0.700 V was (1.94±0.03) mA M−1 (r=0.9991), with a linear range between 0.25 and 5.00 mM, a detection limit of 2.2 μM and a quantification limit of 6.7 μM with minimum interference from lactose (1.5%), maltose (5.7%), galactose (1.2%), ascorbic acid (1.0%), and uric acid (3.3%). The biocatalytic layer demonstrated to be highly reproducible since the R.S.D. for 10 successive amperometric calibrations using the same surface was 3.6%. The sensitivity of the biosensor after 15 day storage at 4 °C remained at 90% of its original value. The combination of the excellent dispersing properties and polycationic nature of polyhistidine, the stability of the MWCNT-Polyhis dispersion, the electrocatalytic properties of MWCNTs, the biocatalytic specificity of GOx, and the permselective properties of Nafion have allowed building up a sensitive, selective, robust, reproducible and stable glucose amperometric biosensor for the quantification of glucose in milk samples." @default.
• W2006312193 created "2016-06-24" @default.
• W2006312193 creator A5011644892 @default.
• W2006312193 creator A5050704659 @default.
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• W2006312193 date "2013-01-01" @default.
• W2006312193 modified "2023-10-08" @default.
• W2006312193 title "Supramolecular architecture based on the self-assembling of multiwall carbon nanotubes dispersed in polyhistidine and glucose oxidase: Characterization and analytical applications for glucose biosensing" @default.
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• W2006312193 doi "https://doi.org/10.1016/j.bios.2012.06.041" @default.
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