FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2006333863> ?p ?o ?g. }

• W2006333863 endingPage "3424" @default.
• W2006333863 startingPage "3413" @default.
• W2006333863 abstract "The Staphylococcus aureus cid and lrg operons play significant roles in the control of autolysis and accumulation of extracellular genomic DNA (eDNA) during biofilm development. Although the molecular mechanisms mediating this control are only beginning to be revealed, it is clear that cell death must be limited to a subfraction of the biofilm population. In the present study, we tested the hypothesis that cid and lrg expression varies during biofilm development as a function of changes in the availability of oxygen. To examine cid and lrg promoter activity during biofilm development, fluorescent reporter fusion strains were constructed and grown in a BioFlux microfluidic system, generating time-lapse epifluorescence images of biofilm formation, which allows the spatial and temporal localization of gene expression. Consistent with cid induction under hypoxic conditions, the cid::gfp fusion strain expressed green fluorescent protein predominantly within the interior of the tower structures, similar to the pattern of expression observed with a strain carrying a gfp fusion to the hypoxia-induced promoter controlling the expression of the lactose dehydrogenase gene. The lrg promoter was also expressed within towers but appeared more diffuse throughout the tower structures, indicating that it was oxygen independent. Unexpectedly, the results also demonstrated the existence of tower structures with different expression phenotypes and physical characteristics, suggesting that these towers exhibit different metabolic activities. Overall, the findings presented here support a model in which oxygen is important in the spatial and temporal control of cid expression within a biofilm and that tower structures formed during biofilm development exhibit metabolically distinct niches." @default.
• W2006333863 created "2016-06-24" @default.
• W2006333863 creator A5005469644 @default.
• W2006333863 creator A5007609770 @default.
• W2006333863 creator A5015665697 @default.
• W2006333863 creator A5016909196 @default.
• W2006333863 creator A5045569473 @default.
• W2006333863 creator A5048881547 @default.
• W2006333863 creator A5049789683 @default.
• W2006333863 creator A5089324530 @default.
• W2006333863 date "2013-06-01" @default.
• W2006333863 modified "2023-10-14" @default.
• W2006333863 title "Use of Microfluidic Technology To Analyze Gene Expression during Staphylococcus aureus Biofilm Formation Reveals Distinct Physiological Niches" @default.
• W2006333863 cites W1530112701 @default.
• W2006333863 cites W1560024199 @default.
• W2006333863 cites W1665252985 @default.
• W2006333863 cites W1914847270 @default.
• W2006333863 cites W1964727305 @default.
• W2006333863 cites W1965158615 @default.
• W2006333863 cites W1967377218 @default.
• W2006333863 cites W1979317235 @default.
• W2006333863 cites W1980618604 @default.
• W2006333863 cites W1981804299 @default.
• W2006333863 cites W1988837048 @default.
• W2006333863 cites W1989087836 @default.
• W2006333863 cites W1991642577 @default.
• W2006333863 cites W1994762646 @default.
• W2006333863 cites W1996064131 @default.
• W2006333863 cites W2003208348 @default.
• W2006333863 cites W2005265983 @default.
• W2006333863 cites W2026220836 @default.
• W2006333863 cites W2040626723 @default.
• W2006333863 cites W2043346930 @default.
• W2006333863 cites W2048044149 @default.
• W2006333863 cites W2053460725 @default.
• W2006333863 cites W2053860875 @default.
• W2006333863 cites W2080410350 @default.
• W2006333863 cites W2082900223 @default.
• W2006333863 cites W2084622748 @default.
• W2006333863 cites W2096973732 @default.
• W2006333863 cites W2110324548 @default.
• W2006333863 cites W2111899305 @default.
• W2006333863 cites W2114570899 @default.
• W2006333863 cites W2114785758 @default.
• W2006333863 cites W2120160920 @default.
• W2006333863 cites W2122589790 @default.
• W2006333863 cites W2126332094 @default.
• W2006333863 cites W2128139766 @default.
• W2006333863 cites W2132160957 @default.
• W2006333863 cites W2132927585 @default.
• W2006333863 cites W2134515577 @default.
• W2006333863 cites W2137670228 @default.
• W2006333863 cites W2138502991 @default.
• W2006333863 cites W2143034026 @default.
• W2006333863 cites W2144821695 @default.
• W2006333863 cites W2145111318 @default.
• W2006333863 cites W2156382695 @default.
• W2006333863 cites W2158709371 @default.
• W2006333863 cites W2159617230 @default.
• W2006333863 cites W2164458860 @default.
• W2006333863 cites W2167240777 @default.
• W2006333863 cites W2169452559 @default.
• W2006333863 cites W4302545197 @default.
• W2006333863 doi "https://doi.org/10.1128/aem.00395-13" @default.
• W2006333863 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3648040" @default.
• W2006333863 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23524683" @default.
• W2006333863 hasPublicationYear "2013" @default.
• W2006333863 type Work @default.
• W2006333863 sameAs 2006333863 @default.
• W2006333863 citedByCount "81" @default.
• W2006333863 countsByYear W20063338632013 @default.
• W2006333863 countsByYear W20063338632014 @default.
• W2006333863 countsByYear W20063338632015 @default.
• W2006333863 countsByYear W20063338632016 @default.
• W2006333863 countsByYear W20063338632017 @default.
• W2006333863 countsByYear W20063338632018 @default.
• W2006333863 countsByYear W20063338632019 @default.
• W2006333863 countsByYear W20063338632020 @default.
• W2006333863 countsByYear W20063338632021 @default.
• W2006333863 countsByYear W20063338632022 @default.
• W2006333863 countsByYear W20063338632023 @default.
• W2006333863 crossrefType "journal-article" @default.
• W2006333863 hasAuthorship W2006333863A5005469644 @default.
• W2006333863 hasAuthorship W2006333863A5007609770 @default.
• W2006333863 hasAuthorship W2006333863A5015665697 @default.
• W2006333863 hasAuthorship W2006333863A5016909196 @default.
• W2006333863 hasAuthorship W2006333863A5045569473 @default.
• W2006333863 hasAuthorship W2006333863A5048881547 @default.
• W2006333863 hasAuthorship W2006333863A5049789683 @default.
• W2006333863 hasAuthorship W2006333863A5089324530 @default.
• W2006333863 hasBestOaLocation W20063338631 @default.
• W2006333863 hasConcept C104317684 @default.
• W2006333863 hasConcept C142613039 @default.
• W2006333863 hasConcept C144024400 @default.
• W2006333863 hasConcept C149923435 @default.
• W2006333863 hasConcept C150194340 @default.
• W2006333863 hasConcept C165864922 @default.
• W2006333863 hasConcept C203075996 @default.

• next