FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2006342208> ?p ?o ?g. }

• W2006342208 endingPage "51" @default.
• W2006342208 startingPage "43" @default.
• W2006342208 abstract "Mycobacterium tuberculosis (MTB) secretory proteins are generally considered important antigens for immune protection against tuberculosis (TB). An 8.3-kDa secretory antigen of MTB and Mycobacterium bovis bacillus Calmette-Guérin (BCG), called SA5K, was recently identified and cloned in our laboratory. In this report, recombinant SA5K containing a histidine hexamer was expressed in Escherichia coli and purified to investigate its biochemical structure and to establish whether it was immunogenic for healthy sensitized and nonsensitized human donors and for patients infected with MTB. The protein nucleotide sequence was shown to be identical in BCG and in MTB. SA5K revealed an abnormal electrophoretic mobility in SDS-PAGE that made it look lighter than it is in Western blotting. While recombinant SA5K was poorly recognized by T lymphocytes from patients with pulmonary TB, it elicited proliferation of CD4+ T lymphocytes in the vast majority of healthy individuals sensitized to mycobacterial antigens by BCG vaccination. At a serum dilution of 1 : 80, antibodies reacting against recombinant SA5K were found in 67% of sera from TB patients and in 73% of sera from healthy subjects. The percentage of positive subjects dropped at higher serum dilutions, but no significant difference in the recognition rate was observed between TB patients and healthy donors and between healthy vaccinated and nonvaccinated subjects. Owing to the high percentage of sera from healthy subjects who recognized SA5K in Western blotting, the antigen seems to exhibit, at least in the present form, a poor specificity for an employment for a serodiagnosis of TB." @default.
• W2006342208 created "2016-06-24" @default.
• W2006342208 creator A5004590272 @default.
• W2006342208 creator A5004721023 @default.
• W2006342208 creator A5010162843 @default.
• W2006342208 creator A5031085787 @default.
• W2006342208 creator A5035579778 @default.
• W2006342208 creator A5043868737 @default.
• W2006342208 creator A5062793956 @default.
• W2006342208 creator A5066636011 @default.
• W2006342208 creator A5066705302 @default.
• W2006342208 date "2002-06-28" @default.
• W2006342208 modified "2023-10-18" @default.
• W2006342208 title "Purification, Biochemical Characterization and Immunogenicity of SA5K, a Secretion Antigen of <i>Mycobacterium tuberculosis</i>" @default.
• W2006342208 cites W1928129263 @default.
• W2006342208 cites W1953458801 @default.
• W2006342208 cites W1966744071 @default.
• W2006342208 cites W1987196776 @default.
• W2006342208 cites W1989533108 @default.
• W2006342208 cites W2038612421 @default.
• W2006342208 cites W2038907536 @default.
• W2006342208 cites W2041179613 @default.
• W2006342208 cites W2067728592 @default.
• W2006342208 cites W2076174684 @default.
• W2006342208 cites W2093700912 @default.
• W2006342208 cites W2096008397 @default.
• W2006342208 cites W2097031247 @default.
• W2006342208 cites W2098811056 @default.
• W2006342208 cites W2101532425 @default.
• W2006342208 cites W2103887556 @default.
• W2006342208 cites W2105917801 @default.
• W2006342208 cites W2116654553 @default.
• W2006342208 cites W2118905950 @default.
• W2006342208 cites W2134907254 @default.
• W2006342208 cites W2136919736 @default.
• W2006342208 cites W2139576494 @default.
• W2006342208 cites W2145578574 @default.
• W2006342208 cites W2154528485 @default.
• W2006342208 cites W2157605618 @default.
• W2006342208 cites W2157737997 @default.
• W2006342208 cites W2161652552 @default.
• W2006342208 cites W2162559009 @default.
• W2006342208 cites W2163318068 @default.
• W2006342208 cites W2166582198 @default.
• W2006342208 cites W2167843072 @default.
• W2006342208 doi "https://doi.org/10.1046/j.1365-3083.2002.01104.x" @default.
• W2006342208 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12100470" @default.
• W2006342208 hasPublicationYear "2002" @default.
• W2006342208 type Work @default.
• W2006342208 sameAs 2006342208 @default.
• W2006342208 citedByCount "8" @default.
• W2006342208 countsByYear W20063422082012 @default.
• W2006342208 crossrefType "journal-article" @default.
• W2006342208 hasAuthorship W2006342208A5004590272 @default.
• W2006342208 hasAuthorship W2006342208A5004721023 @default.
• W2006342208 hasAuthorship W2006342208A5010162843 @default.
• W2006342208 hasAuthorship W2006342208A5031085787 @default.
• W2006342208 hasAuthorship W2006342208A5035579778 @default.
• W2006342208 hasAuthorship W2006342208A5043868737 @default.
• W2006342208 hasAuthorship W2006342208A5062793956 @default.
• W2006342208 hasAuthorship W2006342208A5066636011 @default.
• W2006342208 hasAuthorship W2006342208A5066705302 @default.
• W2006342208 hasBestOaLocation W20063422081 @default.
• W2006342208 hasConcept C104317684 @default.
• W2006342208 hasConcept C142724271 @default.
• W2006342208 hasConcept C147483822 @default.
• W2006342208 hasConcept C159047783 @default.
• W2006342208 hasConcept C159654299 @default.
• W2006342208 hasConcept C203014093 @default.
• W2006342208 hasConcept C22070199 @default.
• W2006342208 hasConcept C2777975735 @default.
• W2006342208 hasConcept C2778497218 @default.
• W2006342208 hasConcept C2780868878 @default.
• W2006342208 hasConcept C2781069245 @default.
• W2006342208 hasConcept C40767141 @default.
• W2006342208 hasConcept C55493867 @default.
• W2006342208 hasConcept C71924100 @default.
• W2006342208 hasConcept C86803240 @default.
• W2006342208 hasConcept C89423630 @default.
• W2006342208 hasConceptScore W2006342208C104317684 @default.
• W2006342208 hasConceptScore W2006342208C142724271 @default.
• W2006342208 hasConceptScore W2006342208C147483822 @default.
• W2006342208 hasConceptScore W2006342208C159047783 @default.
• W2006342208 hasConceptScore W2006342208C159654299 @default.
• W2006342208 hasConceptScore W2006342208C203014093 @default.
• W2006342208 hasConceptScore W2006342208C22070199 @default.
• W2006342208 hasConceptScore W2006342208C2777975735 @default.
• W2006342208 hasConceptScore W2006342208C2778497218 @default.
• W2006342208 hasConceptScore W2006342208C2780868878 @default.
• W2006342208 hasConceptScore W2006342208C2781069245 @default.
• W2006342208 hasConceptScore W2006342208C40767141 @default.
• W2006342208 hasConceptScore W2006342208C55493867 @default.
• W2006342208 hasConceptScore W2006342208C71924100 @default.
• W2006342208 hasConceptScore W2006342208C86803240 @default.
• W2006342208 hasConceptScore W2006342208C89423630 @default.
• W2006342208 hasIssue "1" @default.
• W2006342208 hasLocation W20063422081 @default.
• W2006342208 hasLocation W20063422082 @default.

• next