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- W2006397723 abstract "A heterologous substrate assay for the human immunodeficiency virus type 1 (HIV-1) protease has been engineered inEscherichia coli.This assay detects the activity of the HIV-1 protease within intact bacterial cells and does not require biochemical purification of either the enzyme or the substrate. For this assay, nine HIV-1 protease specificity sites were genetically engineered into a heterologous protein (galactokinase) and the relative processing of these substrates by the wild-type and a substituted HIV-1 protease was determined. The results from these experiments revealed that the activity of the HIV-1 protease in the engineered heterologous substrate assay is consistent with previously reportedin vitroassays andin vivoobservations as well as a proposed catalytic specificity model." @default.
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- W2006397723 date "1996-11-01" @default.
- W2006397723 modified "2023-10-18" @default.
- W2006397723 title "A Heterologous Substrate Assay for the HIV-1 Protease Engineered inEscherichia coli" @default.
- W2006397723 doi "https://doi.org/10.1006/abio.1996.0433" @default.
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