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- W2006422919 abstract "The calcium activated neutral protease from bovine ventricular muscle requires milli-molar concentration of Ca ions for the activation of the proteolysis of troponin-T, troponin-I and tropomyosin. The exogenous protease inhibitors were examined concerning the blocking action of this enzyme. Both leupeptin and antipain were effective for the inhibition at the nearly same molar concentration as the protease. Lineweaver plot for both the protease alone and protease with leupeptin showed straight lines, and the mode of the inhibition was non-competitive type. Natural actomyosin, pretreated with this protease showed markedly reduced sensitivity to Ca ions. With the addition of leupeptin to the pretreatment, however, the Ca sensitivity was well preserved." @default.
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- W2006422919 date "1978-05-01" @default.
- W2006422919 modified "2023-10-09" @default.
- W2006422919 title "Inhibition of proteolytic activity of calcium activated neutral protease by leupeptin and antipain" @default.
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- W2006422919 doi "https://doi.org/10.1016/0006-291x(78)90900-2" @default.
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