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• W2006455220 abstract "Fourteen-day-old rat pituitary tissue represents an attractive model for studying cell population dynamics, particularly of gonadotrophs. Prolonged three-dimensional culture in serum- and hormone-free medium causes a striking decline in somatotroph abundance but a several-fold rise in monohormonal LHβ-positive cell number, whereas bihormonal gonadotrophs almost disappear. In the present study, we investigated whether these changes are inter-related by examining the effects of growth hormone-releasing hormone (GHRH) and glucocorticoids, two protagonist regulators of somatotrophs. Cells were identified by single cell reverse transcriptase-polymerase chain reaction (RT-PCR) and immunofluorescence. Supplementation of the cultures for 2 weeks with GHRH (1 nm) did not augment the proportion of somatotrophs, but expanded the nonhormonal cell population. GHRH reduced the proportion of monohormonal luteinising hormone (LH)β mRNA positive cells to approximately 50% of control, although the effect was not seen when these cells were visualised by immunostaining. Supplementation of the cultures with dexamethasone (4 nm) for 3 weeks partially rescued LHβ/follicle-stimulating hormone β cells and fully rescued the GH mRNA cells in parallel with a decline in nonhormonal cell abundance, but strongly reduced bromodeoxyuridine labelling of GH-immunoreactive cells. As studied by patch-clamp single cell RT-PCR at the start of culture, GHRH caused an acute rise in intracellular [Ca2+] in some monohormonal GH cells, but at a higher incidence in cells expressing LHβ mRNA, alone or in combination with GH mRNA and/or pro-opiomelanocortin (POMC) mRNA. The present data suggest that, in the 14-day-old rat pituitary, the majority of GHRH target cells are cells expressing LHβ mRNA alone or in combination with GH and/or POMC mRNA. The data show co-regulation of gonadotroph and somatotroph population sizes by glucocorticoids and GHRH, with the former preserving bihormonal gonadotrophs and the latter repressing LHβ-only cell abundance. GHRH may not expand the somatotroph population unless glucocorticoid hormone is present to maintain terminal differentiation." @default.
• W2006455220 created "2016-06-24" @default.
• W2006455220 creator A5025934482 @default.
• W2006455220 creator A5059190163 @default.
• W2006455220 creator A5060173638 @default.
• W2006455220 date "2008-05-01" @default.
• W2006455220 modified "2023-09-23" @default.
• W2006455220 title "Growth Hormone-Releasing Hormone and Glucocorticoids Determine the Balance Between Luteinising Hormone (LH) β- and LHβ/Follicle-Stimulating Hormone β-Positive Gonadotrophs and Somatotrophs in the 14-Day-Old Rat Pituitary Tissue in Aggregate Cell Culture" @default.
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• W2006455220 doi "https://doi.org/10.1111/j.1365-2826.2008.01698.x" @default.
• W2006455220 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18363807" @default.
• W2006455220 hasPublicationYear "2008" @default.
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