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- W2006543165 abstract "Abstract Ethylene rapidly and transiently up-regulates the activity of several monomeric GTP-binding proteins (monomeric G proteins) in leaves of Arabidopsis as determined by two-dimensional gel electrophoresis and autoradiographic analyses. The activation is suppressed by the receptor-directed inhibitor 1-methylcyclopropene. In theetr1-1 mutant, constitutive activity of all the monomeric G proteins activated by ethylene is down-regulated relative to wild type, and ethylene treatment has no effect on the levels of activity. Conversely, in the ctr1-1 mutant, several of the monomeric G proteins activated by ethylene are constitutively up-regulated. However, the activation profile of ctr1-1does not exactly mimic that of ethylene-treated wild type. Biochemical and molecular evidence suggested that some of these monomeric G proteins are of the Rab class. Expression of the genes for a number of monomeric G proteins in response to ethylene was investigated by reverse transcriptase-PCR. Rab8 andAra3 expression was increased within 10 min of ethylene treatment, although levels fell back significantly by 40 min. In theetr1-1 mutant, expression of Rab8 was lower than wild type and unaffected by ethylene; inctr1-1, expression of Rab8 was much higher than wild type and comparable with that seen in ethylene treatments. Expression in ctr1-1 was also unaffected by ethylene. Thus, the data indicate a role for monomeric G proteins in ethylene signal transduction." @default.
- W2006543165 created "2016-06-24" @default.
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- W2006543165 date "2003-04-01" @default.
- W2006543165 modified "2023-10-04" @default.
- W2006543165 title "Ethylene Regulates Monomeric GTP-Binding Protein Gene Expression and Activity in Arabidopsis" @default.
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- W2006543165 doi "https://doi.org/10.1104/pp.014035" @default.
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- W2006543165 hasPublicationYear "2003" @default.
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