FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2006593747> ?p ?o ?g. }

• W2006593747 endingPage "1243" @default.
• W2006593747 startingPage "1235" @default.
• W2006593747 abstract "In western North America, Douglas-fir (Pseudotsuga menziesii) is the most economically important conifer species susceptible to laminated root rot caused by Phellinus weirii. While attempting to internally sequence an endochitinase found to be up-regulated in P. weirii-infected Douglas-fir roots, we obtained overlapping peptide fragments showing 28% similarity with a PR-5 thaumatin-like protein (TLP) designated PmTLP (Pm for Pseudotsuga menziesi). A rabbit polyclonal antibody was reared against a synthetic peptide composed of a 29-amino-acid-long, conserved, internal sequence of PmTLP and purified by immunoaffinity. Western immunoblot analysis of infected roots of 24-year-old coastalfir showed significantly higher amounts of PmTLP (P < 0.01) closest to the point of P. weirii inoculation and infection than in uninfected regions of the same root. The antibody was also used to screen for PmTLP in roots of 25-year-old interior Douglas-firs naturally infected with a related pathogen, Armillaria ostoyae, and results showed significantly higher levels of PmTLP in bark tissues adjacent to infection (P < 0.05) than in uninfected tissue. Using polymerase chain reaction (PCR)-based cloning, the cDNA of PmTLP was shown to have a 702-bp open reading frame with a signal peptide cleavage site at 155 bp corresponding to a 29-amino-acid-long residue prior to the start of the N-terminal. Based on the deduced amino acid sequence, the molecular mass of the putative PmTLP was calculated to be 21.0 kDa with an isoelectric point of 3.71. Alignment analysis of PmTLP cDNA with a representative genomic DNA PCR sequence showed presence of one intron of variable size, within the coding region. The induction of PmTLP at the site of root infection and its presence in needle tissue suggests a general role for this protein in adaptation to stress and may be part of an integrated defense response initiated by the host to impede further pathogen spread." @default.
• W2006593747 created "2016-06-24" @default.
• W2006593747 creator A5008568889 @default.
• W2006593747 creator A5058490319 @default.
• W2006593747 creator A5060905942 @default.
• W2006593747 creator A5065073922 @default.
• W2006593747 creator A5074286540 @default.
• W2006593747 date "2004-11-01" @default.
• W2006593747 modified "2023-09-26" @default.
• W2006593747 title "Gene Cloning and Tissue Expression Analysis of a PR-5 Thaumatin-Like Protein in <i>Phellinus weirii</i>-Infected Douglas-Fir" @default.
• W2006593747 cites W1517917786 @default.
• W2006593747 cites W1539153888 @default.
• W2006593747 cites W1976886929 @default.
• W2006593747 cites W1980821454 @default.
• W2006593747 cites W1988704245 @default.
• W2006593747 cites W1989145266 @default.
• W2006593747 cites W1991837864 @default.
• W2006593747 cites W2000372894 @default.
• W2006593747 cites W2007351562 @default.
• W2006593747 cites W2008898015 @default.
• W2006593747 cites W2011428634 @default.
• W2006593747 cites W2020706816 @default.
• W2006593747 cites W2036088949 @default.
• W2006593747 cites W2038163496 @default.
• W2006593747 cites W2041942712 @default.
• W2006593747 cites W2055848303 @default.
• W2006593747 cites W2088447195 @default.
• W2006593747 cites W2089298322 @default.
• W2006593747 cites W2090393214 @default.
• W2006593747 cites W2106882534 @default.
• W2006593747 cites W2112411709 @default.
• W2006593747 cites W2120231778 @default.
• W2006593747 cites W2142887538 @default.
• W2006593747 cites W2158714788 @default.
• W2006593747 cites W2168453728 @default.
• W2006593747 cites W2169919412 @default.
• W2006593747 cites W2170217346 @default.
• W2006593747 cites W2171091522 @default.
• W2006593747 cites W3142042565 @default.
• W2006593747 cites W4211017549 @default.
• W2006593747 cites W88178131 @default.
• W2006593747 cites W96653237 @default.
• W2006593747 doi "https://doi.org/10.1094/phyto.2004.94.11.1235" @default.
• W2006593747 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18944459" @default.
• W2006593747 hasPublicationYear "2004" @default.
• W2006593747 type Work @default.
• W2006593747 sameAs 2006593747 @default.
• W2006593747 citedByCount "21" @default.
• W2006593747 countsByYear W20065937472013 @default.
• W2006593747 countsByYear W20065937472015 @default.
• W2006593747 countsByYear W20065937472019 @default.
• W2006593747 crossrefType "journal-article" @default.
• W2006593747 hasAuthorship W2006593747A5008568889 @default.
• W2006593747 hasAuthorship W2006593747A5058490319 @default.
• W2006593747 hasAuthorship W2006593747A5060905942 @default.
• W2006593747 hasAuthorship W2006593747A5065073922 @default.
• W2006593747 hasAuthorship W2006593747A5074286540 @default.
• W2006593747 hasBestOaLocation W20065937471 @default.
• W2006593747 hasConcept C104317684 @default.
• W2006593747 hasConcept C10858879 @default.
• W2006593747 hasConcept C153911025 @default.
• W2006593747 hasConcept C159654299 @default.
• W2006593747 hasConcept C167625842 @default.
• W2006593747 hasConcept C187882448 @default.
• W2006593747 hasConcept C2779769909 @default.
• W2006593747 hasConcept C47289529 @default.
• W2006593747 hasConcept C515207424 @default.
• W2006593747 hasConcept C54355233 @default.
• W2006593747 hasConcept C55493867 @default.
• W2006593747 hasConcept C61053724 @default.
• W2006593747 hasConcept C86803240 @default.
• W2006593747 hasConcept C956191 @default.
• W2006593747 hasConceptScore W2006593747C104317684 @default.
• W2006593747 hasConceptScore W2006593747C10858879 @default.
• W2006593747 hasConceptScore W2006593747C153911025 @default.
• W2006593747 hasConceptScore W2006593747C159654299 @default.
• W2006593747 hasConceptScore W2006593747C167625842 @default.
• W2006593747 hasConceptScore W2006593747C187882448 @default.
• W2006593747 hasConceptScore W2006593747C2779769909 @default.
• W2006593747 hasConceptScore W2006593747C47289529 @default.
• W2006593747 hasConceptScore W2006593747C515207424 @default.
• W2006593747 hasConceptScore W2006593747C54355233 @default.
• W2006593747 hasConceptScore W2006593747C55493867 @default.
• W2006593747 hasConceptScore W2006593747C61053724 @default.
• W2006593747 hasConceptScore W2006593747C86803240 @default.
• W2006593747 hasConceptScore W2006593747C956191 @default.
• W2006593747 hasIssue "11" @default.
• W2006593747 hasLocation W20065937471 @default.
• W2006593747 hasLocation W20065937472 @default.
• W2006593747 hasOpenAccess W2006593747 @default.
• W2006593747 hasPrimaryLocation W20065937471 @default.
• W2006593747 hasRelatedWork W1481240978 @default.
• W2006593747 hasRelatedWork W2006809442 @default.
• W2006593747 hasRelatedWork W2042715886 @default.
• W2006593747 hasRelatedWork W2060427643 @default.
• W2006593747 hasRelatedWork W2351266571 @default.
• W2006593747 hasRelatedWork W2369429805 @default.
• W2006593747 hasRelatedWork W2373282699 @default.

• next