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• W2006607562 abstract "This paper describes the use of a cuvette-based surface plasmon resonance (SPR) instrument to measure biocatalyzed precipitation reactions. Enzyme-modified SPR sensor disk forms the base of a cuvette, in which the substrate solution is added with stirring. The determination of the substrate concentration relies on the measurement of SPR angle shift (Deltatheta(SPR)) induced by the deposition of the insoluble products without involving in any electrochemical reactions. As examples, horseradish peroxidase (HRP)-modified monoenzyme SPR sensor and HRP-glucose oxidase bienzyme-layered sensor are created to determine hydrogen peroxide and glucose via the catalyzed oxidation of 4-chloro-1-naphthol (4-CN). The deposition of the oxidized 4-CN-insoluble products leads to SPR angle shifts, which are linear to H(2)O(2) and glucose in the concentration ranges of 0.067-7.24 x 10(-5) and 0.7-8.3 x 10(-4) mM, respectively. The SPR sensitivities are greater than those of nonelectrochemical quartz crystal microbalance (QCM) (the parallel results in this study) and compare favorable with those of electrochemical QCM and electrochemical SPR methods. This study opens the field for enhanced SPR measurements by using biocatalyzed precipitation as a signal amplification method." @default.
• W2006607562 created "2016-06-24" @default.
• W2006607562 creator A5033345953 @default.
• W2006607562 creator A5051992229 @default.
• W2006607562 date "2001-12-01" @default.
• W2006607562 modified "2023-10-16" @default.
• W2006607562 title "Determination of Monoenzyme- and Bienzyme-Stimulated Precipitation by a Cuvette-Based Surface Plasmon Resonance Instrument" @default.
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• W2006607562 doi "https://doi.org/10.1006/abio.2001.5429" @default.
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