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• W2006634545 abstract "ABSTRACT Current hepatitis E virus (HEV) negative-sense RNA detection assays have the drawback of false positivity. cDNA synthesis using tag-based primer and Superscript RT-III followed by exonuclease I treatment increased the specificity. Assays could detect as few as 10 copies of negative-sense RNA and could be used in detecting low levels of HEV replication in cells. Virus particles in stool samples of hepatitis E patients showed encapsidation of negative-sense RNA along with HEV genomic RNA." @default.
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• W2006634545 date "2012-04-01" @default.
• W2006634545 modified "2023-09-27" @default.
• W2006634545 title "Detection of Negative-Sense RNA in Packaged Hepatitis E Virions by Use of an Improved Strand-Specific Reverse Transcription-PCR Method" @default.
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• W2006634545 doi "https://doi.org/10.1128/jcm.06717-11" @default.
• W2006634545 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3318542" @default.
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