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- W2006642801 abstract "It is well established that endothelial dysfunction contributes to ischemia-reperfusion injury of cardiovascular system. This phenomenon can be limited by the ischemic preconditioning. Recently, it was shown that mitochondrial ATP regulated potassium channel activation induced ischemic preconditiong of the endothelium in humans in vivo.In our study a single channel activity was measured after patch-clamp of the mitoplasts isolated from endothelial cell line (EA.hy926). Mitoplast samples were prepared by addition to a hypotonic solution causing the cristae of the inner membrane to unfold and breaking of the outer membrane. Isotonicity was restored by adding of hypertonic solution. A potassium selective current was recorded with mean conductance 270 ± 10 pS in symmetrical 150 mM KCl solution. Patch-clamp single channel studies showed properties of the large conductance Ca2+−regulated potassium channel (BKCa channel): it was activated by calcium and NS1619 an activator of BKCa channel at micromolar concentration range. These effects were blocked irreversible by iberiotoxin (IbTx), an inhibitor of BKCa channel. Additionally, we showed that inhibitor of mitoKATP channel (ATP/Mg2+ complex) have no effects on observed activity of ion channel.We conclude that large conductance Ca2+−regulated potassium channels are present in mitochondria isolated from endothelial cell line.This work was supported by Polish Mitochondrial Network MitoNet.pl and grant N30105331/1676" @default.
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- W2006642801 date "2009-02-01" @default.
- W2006642801 modified "2023-09-28" @default.
- W2006642801 title "Large Conductance Potassium Channel In Mitochondria of Endothelial Cell" @default.
- W2006642801 doi "https://doi.org/10.1016/j.bpj.2008.12.2776" @default.
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