FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2006648085> ?p ?o ?g. }

• W2006648085 endingPage "487" @default.
• W2006648085 startingPage "479" @default.
• W2006648085 abstract "The platelet-derived growth factor (PDGF) family of ligands (composed of A-, B-, C-, and D-chains), potent mitogens, and chemoattractants for cells of mesenchymal origin has been implicated in numerous vascular pathologies involving smooth muscle cell (SMC) hyperplasia. Understanding the molecular mechanisms mediating PDGF transcription would provide new insights into strategies to control PDGF-dependent pathophysiologic processes. We demonstrated previously that PDGF-A expression is under the positive regulatory influence of Sp1, Sp3, and Egr-1 and is negatively controlled by GCF2, NF-1(X), and WT-1. In this article, we demonstrate that Ets-1 induces PDGF-A expression in primary rat aortic SMCs at the level of transcription and mRNA expression. Electrophoretic mobility shift, supershift, and mutational analyses revealed a functional role for the −555 TTCC −552 motif in the PDGF-A promoter that binds endogenous Ets-1. Chromatin immunoprecipitation analysis showed the interaction of endogenous and exogenous Ets-1 or glutathione S -transferase-tagged Ets-1, bearing only the DNA-binding domain with the authentic PDGF-A promoter. Conversely, dominant-negative mutant of Ets-1 blocked the promoter interaction of endogenous Ets-1. Overexpression of Ets-1 but not the mutant form of Ets-1 activates the PDGF-A promoter cooperatively with Sp1. Sp1, which interacts with Ets-1, failed to induce PDGF-A promoter-dependent expression if the promoter contained a site-specific mutation in this novel Ets-binding site. Small interfering RNA to Ets-1 and Sp1 blocked PDGF-BB- and serum-inducible PDGF-A expression. SMC growth was stimulated by Ets-1 and Sp1 separately and further increased by both factors together. Ets-1-inducible mitogenesis is blocked by antibodies neutralizing PDGF-A and involves activation of the PDGF α-receptor, which binds PDGF-A. These findings identify a functional cis -acting element for Ets-1 in the PDGF-A promoter and demonstrate that Sp1 and Ets-1 cooperatively activate PDGF-A transcription in vascular SMCs." @default.
• W2006648085 created "2016-06-24" @default.
• W2006648085 creator A5012564439 @default.
• W2006648085 creator A5065860849 @default.
• W2006648085 date "2004-09-03" @default.
• W2006648085 modified "2023-10-13" @default.
• W2006648085 title "Ets-1 Stimulates Platelet-Derived Growth Factor A-Chain Gene Transcription and Vascular Smooth Muscle Cell Growth via Cooperative Interactions With Sp1" @default.
• W2006648085 cites W1483090589 @default.
• W2006648085 cites W1597241698 @default.
• W2006648085 cites W1703816965 @default.
• W2006648085 cites W1971664488 @default.
• W2006648085 cites W1998109615 @default.
• W2006648085 cites W2000100672 @default.
• W2006648085 cites W2001381510 @default.
• W2006648085 cites W2002405735 @default.
• W2006648085 cites W2008468198 @default.
• W2006648085 cites W2011957331 @default.
• W2006648085 cites W2013850554 @default.
• W2006648085 cites W2029978834 @default.
• W2006648085 cites W2037728756 @default.
• W2006648085 cites W2042524894 @default.
• W2006648085 cites W2055220950 @default.
• W2006648085 cites W2064688277 @default.
• W2006648085 cites W2070794112 @default.
• W2006648085 cites W2083757724 @default.
• W2006648085 cites W2093210324 @default.
• W2006648085 cites W2095353118 @default.
• W2006648085 cites W2097146615 @default.
• W2006648085 cites W2120789358 @default.
• W2006648085 cites W2122719162 @default.
• W2006648085 cites W2149442576 @default.
• W2006648085 cites W2151045797 @default.
• W2006648085 cites W2183745781 @default.
• W2006648085 cites W2341350186 @default.
• W2006648085 cites W74408211 @default.
• W2006648085 doi "https://doi.org/10.1161/01.res.0000141135.36279.67" @default.
• W2006648085 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15297375" @default.
• W2006648085 hasPublicationYear "2004" @default.
• W2006648085 type Work @default.
• W2006648085 sameAs 2006648085 @default.
• W2006648085 citedByCount "46" @default.
• W2006648085 countsByYear W20066480852012 @default.
• W2006648085 countsByYear W20066480852013 @default.
• W2006648085 countsByYear W20066480852014 @default.
• W2006648085 countsByYear W20066480852015 @default.
• W2006648085 countsByYear W20066480852016 @default.
• W2006648085 countsByYear W20066480852017 @default.
• W2006648085 countsByYear W20066480852019 @default.
• W2006648085 countsByYear W20066480852021 @default.
• W2006648085 crossrefType "journal-article" @default.
• W2006648085 hasAuthorship W2006648085A5012564439 @default.
• W2006648085 hasAuthorship W2006648085A5065860849 @default.
• W2006648085 hasBestOaLocation W20066480851 @default.
• W2006648085 hasConcept C101762097 @default.
• W2006648085 hasConcept C104317684 @default.
• W2006648085 hasConcept C134018914 @default.
• W2006648085 hasConcept C134320426 @default.
• W2006648085 hasConcept C150194340 @default.
• W2006648085 hasConcept C153911025 @default.
• W2006648085 hasConcept C170493617 @default.
• W2006648085 hasConcept C180361614 @default.
• W2006648085 hasConcept C27153228 @default.
• W2006648085 hasConcept C2775960820 @default.
• W2006648085 hasConcept C2779395532 @default.
• W2006648085 hasConcept C2781294074 @default.
• W2006648085 hasConcept C2992686903 @default.
• W2006648085 hasConcept C54355233 @default.
• W2006648085 hasConcept C57467964 @default.
• W2006648085 hasConcept C62112901 @default.
• W2006648085 hasConcept C81350143 @default.
• W2006648085 hasConcept C86339819 @default.
• W2006648085 hasConcept C86803240 @default.
• W2006648085 hasConcept C95444343 @default.
• W2006648085 hasConceptScore W2006648085C101762097 @default.
• W2006648085 hasConceptScore W2006648085C104317684 @default.
• W2006648085 hasConceptScore W2006648085C134018914 @default.
• W2006648085 hasConceptScore W2006648085C134320426 @default.
• W2006648085 hasConceptScore W2006648085C150194340 @default.
• W2006648085 hasConceptScore W2006648085C153911025 @default.
• W2006648085 hasConceptScore W2006648085C170493617 @default.
• W2006648085 hasConceptScore W2006648085C180361614 @default.
• W2006648085 hasConceptScore W2006648085C27153228 @default.
• W2006648085 hasConceptScore W2006648085C2775960820 @default.
• W2006648085 hasConceptScore W2006648085C2779395532 @default.
• W2006648085 hasConceptScore W2006648085C2781294074 @default.
• W2006648085 hasConceptScore W2006648085C2992686903 @default.
• W2006648085 hasConceptScore W2006648085C54355233 @default.
• W2006648085 hasConceptScore W2006648085C57467964 @default.
• W2006648085 hasConceptScore W2006648085C62112901 @default.
• W2006648085 hasConceptScore W2006648085C81350143 @default.
• W2006648085 hasConceptScore W2006648085C86339819 @default.
• W2006648085 hasConceptScore W2006648085C86803240 @default.
• W2006648085 hasConceptScore W2006648085C95444343 @default.
• W2006648085 hasIssue "5" @default.
• W2006648085 hasLocation W20066480851 @default.
• W2006648085 hasLocation W20066480852 @default.
• W2006648085 hasOpenAccess W2006648085 @default.
• W2006648085 hasPrimaryLocation W20066480851 @default.

• next