FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2006705691> ?p ?o ?g. }

• W2006705691 endingPage "e85498" @default.
• W2006705691 startingPage "e85498" @default.
• W2006705691 abstract "Integrin-linked kinase (ILK) is an important signaling regulator that assembles into the heteroternary complex with adaptor proteins PINCH and parvin (termed the IPP complex). We recently reported that ILK is important for integrin activation in a Chinese hamster ovary (CHO) cell system. We previously established parental CHO cells expressing a constitutively active chimeric integrin (αIIbα6Bβ3) and mutant CHO cells expressing inactive αIIbα6Bβ3 due to ILK deficiency. In this study, we further investigated the underlying mechanisms for ILK-dependent integrin activation. ILK-deficient mutant cells had trace levels of PINCH and α-parvin, and transfection of ILK cDNA into the mutant cells increased not only ILK but also PINCH and α-parvin, resulting in the restoration of αIIbα6Bβ3 activation. In the parental cells expressing active αIIbα6Bβ3, ILK, PINCH, and α-parvin were co-immunoprecipitated, indicating the formation of the IPP complex. Moreover, short interfering RNA (siRNA) experiments targeting PINCH-1 or both α- and β-parvin mRNA in the parent cells impaired the αIIbα6Bβ3 activation as well as the expression of the other components of the IPP complex. In addition, ILK mutants possessing defects in either PINCH or parvin binding failed to restore αIIbα6Bβ3 activation in the mutant cells. Kindlin-2 siRNA in the parental cells impaired αIIbα6Bβ3 activation without disturbing the expression of ILK. For CHO cells stably expressing wild-type αIIbβ3 that is an inactive form, overexpression of a talin head domain (THD) induced αIIbβ3 activation and the THD-induced αIIbβ3 activation was impaired by ILK siRNA through a significant reduction in the expression of the IPP complex. In contrast, overexpression of all IPP components in the αIIbβ3-expressing CHO cells further augmented THD-induced αIIbβ3 activation, whereas they did not induce αIIbβ3 activation without THD. These data suggest that the IPP complex rather than ILK plays an important role and supports integrin activation probably through stabilization of the active conformation." @default.
• W2006705691 created "2016-06-24" @default.
• W2006705691 creator A5009524133 @default.
• W2006705691 creator A5015658424 @default.
• W2006705691 creator A5050278092 @default.
• W2006705691 creator A5052898533 @default.
• W2006705691 creator A5073007367 @default.
• W2006705691 date "2013-12-23" @default.
• W2006705691 modified "2023-10-14" @default.
• W2006705691 title "The Integrin-Linked Kinase-PINCH-Parvin Complex Supports Integrin αIIbβ3 Activation" @default.
• W2006705691 cites W1576773659 @default.
• W2006705691 cites W1602125203 @default.
• W2006705691 cites W1839628238 @default.
• W2006705691 cites W1916715636 @default.
• W2006705691 cites W1986055287 @default.
• W2006705691 cites W1986476951 @default.
• W2006705691 cites W1998357792 @default.
• W2006705691 cites W1998539678 @default.
• W2006705691 cites W2008463822 @default.
• W2006705691 cites W2009912270 @default.
• W2006705691 cites W2019133761 @default.
• W2006705691 cites W2021053981 @default.
• W2006705691 cites W2024873935 @default.
• W2006705691 cites W2028305453 @default.
• W2006705691 cites W2032348507 @default.
• W2006705691 cites W2036248412 @default.
• W2006705691 cites W2038908984 @default.
• W2006705691 cites W2040095760 @default.
• W2006705691 cites W2040379229 @default.
• W2006705691 cites W2046737719 @default.
• W2006705691 cites W2058624823 @default.
• W2006705691 cites W2060136332 @default.
• W2006705691 cites W2069986267 @default.
• W2006705691 cites W2070526130 @default.
• W2006705691 cites W2079892535 @default.
• W2006705691 cites W2087472895 @default.
• W2006705691 cites W2088544660 @default.
• W2006705691 cites W2090659127 @default.
• W2006705691 cites W2093835420 @default.
• W2006705691 cites W2094385181 @default.
• W2006705691 cites W2095504560 @default.
• W2006705691 cites W2097414678 @default.
• W2006705691 cites W2104013179 @default.
• W2006705691 cites W2104839879 @default.
• W2006705691 cites W2108496308 @default.
• W2006705691 cites W2108810454 @default.
• W2006705691 cites W2110731221 @default.
• W2006705691 cites W2115158250 @default.
• W2006705691 cites W2118758577 @default.
• W2006705691 cites W2124943739 @default.
• W2006705691 cites W2125861553 @default.
• W2006705691 cites W2144835558 @default.
• W2006705691 cites W2145339711 @default.
• W2006705691 cites W2148062709 @default.
• W2006705691 cites W2152033018 @default.
• W2006705691 cites W2152800355 @default.
• W2006705691 cites W2157330541 @default.
• W2006705691 cites W2165799676 @default.
• W2006705691 cites W2167033631 @default.
• W2006705691 cites W2167696360 @default.
• W2006705691 cites W2258622567 @default.
• W2006705691 doi "https://doi.org/10.1371/journal.pone.0085498" @default.
• W2006705691 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3871693" @default.
• W2006705691 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24376884" @default.
• W2006705691 hasPublicationYear "2013" @default.
• W2006705691 type Work @default.
• W2006705691 sameAs 2006705691 @default.
• W2006705691 citedByCount "21" @default.
• W2006705691 countsByYear W20067056912014 @default.
• W2006705691 countsByYear W20067056912015 @default.
• W2006705691 countsByYear W20067056912016 @default.
• W2006705691 countsByYear W20067056912017 @default.
• W2006705691 countsByYear W20067056912020 @default.
• W2006705691 countsByYear W20067056912021 @default.
• W2006705691 countsByYear W20067056912022 @default.
• W2006705691 crossrefType "journal-article" @default.
• W2006705691 hasAuthorship W2006705691A5009524133 @default.
• W2006705691 hasAuthorship W2006705691A5015658424 @default.
• W2006705691 hasAuthorship W2006705691A5050278092 @default.
• W2006705691 hasAuthorship W2006705691A5052898533 @default.
• W2006705691 hasAuthorship W2006705691A5073007367 @default.
• W2006705691 hasBestOaLocation W20067056911 @default.
• W2006705691 hasConcept C1491633281 @default.
• W2006705691 hasConcept C153911025 @default.
• W2006705691 hasConcept C170493617 @default.
• W2006705691 hasConcept C175656101 @default.
• W2006705691 hasConcept C184235292 @default.
• W2006705691 hasConcept C185592680 @default.
• W2006705691 hasConcept C195687474 @default.
• W2006705691 hasConcept C22615655 @default.
• W2006705691 hasConcept C54009773 @default.
• W2006705691 hasConcept C54355233 @default.
• W2006705691 hasConcept C55493867 @default.
• W2006705691 hasConcept C66417403 @default.
• W2006705691 hasConcept C81885089 @default.
• W2006705691 hasConcept C82495950 @default.
• W2006705691 hasConcept C86803240 @default.
• W2006705691 hasConcept C95444343 @default.

• next