Matches in SemOpenAlex for { <https://semopenalex.org/work/W2006709849> ?p ?o ?g. }
- W2006709849 abstract "Recent developments in genetically encoded indicators of neural activity (GINAs) have greatly advanced the field of systems neuroscience. As they are encoded by DNA, GINAs can be targeted to genetically defined cellular populations. Combined with fluorescence microscopy, most notably multi-photon imaging, GINAs allow chronic simultaneous optical recordings from large populations of neurons or glial cells in awake, behaving mammals, particularly rodents. This large-scale recording of neural activity at multiple temporal and spatial scales has greatly advanced our understanding of the dynamics of neural circuitry underlying behavior—a critical first step toward understanding the complexities of brain function, such as sensorimotor integration and learning. Here, we summarize the recent development and applications of the major classes of GINAs. In particular, we take an in-depth look at the design of available GINA families with a particular focus on genetically encoded calcium indicators, sensors probing synaptic activity, and genetically encoded voltage indicators. Using the family of the genetically encoded calcium indicator GCaMP as an example, we review established sensor optimization pipelines. We also discuss practical considerations for end users of GINAs about experimental methods including approaches for gene delivery, imaging system requirements, and data analysis techniques. With the growing toolbox of GINAs and with new microscopy techniques pushing beyond their current limits, the age of light can finally achieve the goal of broad and dense sampling of neuronal activity across time and brain structures to obtain a dynamic picture of brain function." @default.
- W2006709849 created "2016-06-24" @default.
- W2006709849 creator A5009751100 @default.
- W2006709849 creator A5078715944 @default.
- W2006709849 creator A5080490976 @default.
- W2006709849 date "2014-12-05" @default.
- W2006709849 modified "2023-10-16" @default.
- W2006709849 title "Monitoring activity in neural circuits with genetically encoded indicators" @default.
- W2006709849 cites W1485458577 @default.
- W2006709849 cites W149497181 @default.
- W2006709849 cites W1514853588 @default.
- W2006709849 cites W1541298265 @default.
- W2006709849 cites W1542263802 @default.
- W2006709849 cites W1571164133 @default.
- W2006709849 cites W1573273475 @default.
- W2006709849 cites W178619301 @default.
- W2006709849 cites W1964440976 @default.
- W2006709849 cites W1965279035 @default.
- W2006709849 cites W1966187182 @default.
- W2006709849 cites W1966264583 @default.
- W2006709849 cites W1966605404 @default.
- W2006709849 cites W1966652252 @default.
- W2006709849 cites W1967100281 @default.
- W2006709849 cites W1967162609 @default.
- W2006709849 cites W1968069163 @default.
- W2006709849 cites W1969326379 @default.
- W2006709849 cites W1969581435 @default.
- W2006709849 cites W1970481007 @default.
- W2006709849 cites W1970499295 @default.
- W2006709849 cites W1971978102 @default.
- W2006709849 cites W1972543620 @default.
- W2006709849 cites W1972869628 @default.
- W2006709849 cites W1976485053 @default.
- W2006709849 cites W1977514547 @default.
- W2006709849 cites W1977744560 @default.
- W2006709849 cites W1977906889 @default.
- W2006709849 cites W1980152736 @default.
- W2006709849 cites W1980726951 @default.
- W2006709849 cites W1982277188 @default.
- W2006709849 cites W1982918139 @default.
- W2006709849 cites W1982969769 @default.
- W2006709849 cites W1984098851 @default.
- W2006709849 cites W1984214088 @default.
- W2006709849 cites W1987278178 @default.
- W2006709849 cites W1989359161 @default.
- W2006709849 cites W1989452564 @default.
- W2006709849 cites W1989470236 @default.
- W2006709849 cites W1990030517 @default.
- W2006709849 cites W1990076574 @default.
- W2006709849 cites W1992028186 @default.
- W2006709849 cites W1993778994 @default.
- W2006709849 cites W1994434699 @default.
- W2006709849 cites W1994647098 @default.
- W2006709849 cites W1994982500 @default.
- W2006709849 cites W1995360097 @default.
- W2006709849 cites W1995375772 @default.
- W2006709849 cites W1996706043 @default.
- W2006709849 cites W1996744375 @default.
- W2006709849 cites W1997529300 @default.
- W2006709849 cites W1998337135 @default.
- W2006709849 cites W2002735853 @default.
- W2006709849 cites W2003456064 @default.
- W2006709849 cites W2005725359 @default.
- W2006709849 cites W2006602134 @default.
- W2006709849 cites W2007495909 @default.
- W2006709849 cites W2008643711 @default.
- W2006709849 cites W2009793830 @default.
- W2006709849 cites W2010002568 @default.
- W2006709849 cites W2011362044 @default.
- W2006709849 cites W2012491533 @default.
- W2006709849 cites W2013151879 @default.
- W2006709849 cites W2013422844 @default.
- W2006709849 cites W2013455564 @default.
- W2006709849 cites W2014015681 @default.
- W2006709849 cites W2015147756 @default.
- W2006709849 cites W2015741711 @default.
- W2006709849 cites W2016166286 @default.
- W2006709849 cites W2017838186 @default.
- W2006709849 cites W2021692017 @default.
- W2006709849 cites W2025282542 @default.
- W2006709849 cites W2027542725 @default.
- W2006709849 cites W2029261862 @default.
- W2006709849 cites W2029932526 @default.
- W2006709849 cites W2032554925 @default.
- W2006709849 cites W2032671105 @default.
- W2006709849 cites W2033708119 @default.
- W2006709849 cites W2035494240 @default.
- W2006709849 cites W2038123362 @default.
- W2006709849 cites W2038171794 @default.
- W2006709849 cites W2041199992 @default.
- W2006709849 cites W2041656098 @default.
- W2006709849 cites W2043274097 @default.
- W2006709849 cites W2044286534 @default.
- W2006709849 cites W2044546215 @default.
- W2006709849 cites W2045914027 @default.
- W2006709849 cites W2046031057 @default.
- W2006709849 cites W2047125104 @default.
- W2006709849 cites W2047483303 @default.
- W2006709849 cites W2052971556 @default.
- W2006709849 cites W2053120786 @default.