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• W2006737265 endingPage "2408" @default.
• W2006737265 startingPage "2400" @default.
• W2006737265 abstract "ABSTRACT The carbenicillin, gentamicin, kanamycin, streptomycin, spectinomycin, sulfonamide, and tobramycin resistance determinants found on Pseudomonas aeruginosa plasmid R151 have previously been shown to translocate to another plasmid, R388, and it was inferred that a transposon, Tn 1404 , carried the resistance determinants. Sequencing of the cassette array from the plasmid known as R388::Tn 1404 revealed two known gene cassettes, oxa10 and aadB , and a previously unidentified cassette determining resistance to streptomycin and spectinomycin, here designated aadA10 , in the order oxa10 - aadB-aadA10. These cassettes replaced the dfrB2 - orfA cassette array of R388, indicating that movement of the resistance determinants from R151 to R388 resulted from recombinational exchange between two class 1 integrons rather than transposition. The AadA10 protein is most closely related to AadA6 (85% identical) and AadA7 (80% identical). The aadA10 cassette found here has only a simple site containing a 7-bp spacer derived from attI1 in place of a 59-base element and is likely to represent a derivative of the complete cassette. IntI1-mediated deletion of the aadA10 cassette was not detected, indicating that this single simple site is either inactive or only weakly active." @default.
• W2006737265 created "2016-06-24" @default.
• W2006737265 creator A5037590671 @default.
• W2006737265 creator A5047347058 @default.
• W2006737265 creator A5080471486 @default.
• W2006737265 date "2002-08-01" @default.
• W2006737265 modified "2023-10-09" @default.
• W2006737265 title "Class 1 Integron Containing a New Gene Cassette, <i>aadA10</i> , Associated with Tn <i>1404</i> from R151" @default.
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• W2006737265 doi "https://doi.org/10.1128/aac.46.8.2400-2408.2002" @default.
• W2006737265 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/127381" @default.
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