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• W2006775504 abstract "<h2>Abstract</h2> Phospholipase A₂ (PLA₂) activity from adult hamster lung was characterized using L-α-1-palmitoyl-2-arachidonyl-[arachidonyl-1-¹⁴C]-phosphatidylcholine as the substrate. The released [¹⁴C]-arachidonic acid was separated by TLC. The enzyme activity increased with increasing incubation time (0–120 minutes), calcium ion concentration (0–25.0 mM) and protein (0–2.0 mg). The optimum pH was 8.0. Deoxycholate had a concentration dependent (0.1 to 0.5 mM) inhibitory effect on the activity. PLA₂ specific activity was the highest in mitochondrial fraction. PLA₂ activity following incubation with bleomycin was increased in a dose related fashion. <i>In vivo</i> study showed that both PLA₂ activity and collagen content in hamster lung were significantly elevated at 14 days followed intratracheal instillation of bleomycin. The activation of PLA₂ may play an important role in bleomycin-induced pulmonary toxicity." @default.
• W2006775504 created "2016-06-24" @default.
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• W2006775504 date "1989-05-01" @default.
• W2006775504 modified "2023-09-24" @default.
• W2006775504 title "Characterization of a phospholipase A2 in hamster lung and in vitro and in vivo effects of bleomycin on this enzyme" @default.
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• W2006775504 doi "https://doi.org/10.1016/0952-3278(89)90023-9" @default.
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