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• W2006777319 abstract "Concanavalin A-induced proliferation of rat T-lymphocytes is completely inhibited by 10(-5) M pyrazofurin, a potent inhibitor of pyrimidine de novo synthesis, as judged by cell viability and [3H]thymidine incorporation. Proliferation is completely restored by 5 X 10(-5) M uridine. Cytidine, deoxycytidine, deoxyuridine and thymidine 10 X 10(-5) M each, fail to re-establish proliferation but produce an isotropic dilution of [3H]thymidine uptake in DNA. Bases (cytosine, uracil and thymine) neither restore proliferation nor induce isotopic dilution. The unexpected inability of cytidine to reverse de novo pyrimidine synthesis inhibition suggests a lack of cytidine deaminase activity in rat T-lymphocytes. This is confirmed by a direct sensitive radioisotopic assay (less than 0.001 nmol X min-1 X 10(-6) cells)." @default.
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• W2006777319 date "1982-11-01" @default.
• W2006777319 modified "2023-09-27" @default.
• W2006777319 title "Uridine as the only alternative to pyrimidine de novo synthesis in rat T lymphocytes" @default.
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• W2006777319 doi "https://doi.org/10.1016/0014-5793(82)81259-3" @default.
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