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- W2006785673 abstract "Binding of dodecyltrimethylammonium cation to calf thymus DNA was studied by using the surfactant-selective electrode as a function of added electrolyte concentration at various temperatures. Binding is highly cooperative and biphasic. An abruptly occurring binding attains a slight shoulder within a very narrow equilibrium concentration range followed by a further augmentation in binding, as the equilibrium concentration is increased. Temperature caused relatively small shift in binding isotherm, but a distinct inversion point was found around 27 °C. Above this temperature the isotherm shifts to the left and then to the right below it. Analogous binding studies as above were repeated with heat-denatured DNA, which binds the surfactant with higher intrinsic affinity but less cooperatively than the intact DNA. A fluorescent probe, pyrene, was employed to assess the microenvironmental polarity of pyrene in DNA before and after surfactant binding. The surfactant–DNA complex has a hydrophobic region akin to ordinary micelle." @default.
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- W2006785673 date "1987-01-01" @default.
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- W2006785673 title "Interaction between Dodecyltrimethylammonium Chloride and DNA" @default.
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- W2006785673 doi "https://doi.org/10.1246/bcsj.60.43" @default.
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