FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2006793940> ?p ?o ?g. }

• W2006793940 endingPage "276" @default.
• W2006793940 startingPage "269" @default.
• W2006793940 abstract "The human embryonic kidney (HEK) 293 cell line is widely used in cell biology research. Although HEK293 cells have been meticulously studied, our knowledge about endogenous G protein-coupled receptors (GPCR) in these cells is incomplete. While studying the effects of bradykinin (BK), a potent growth factor for renal cells, we unexpectedly discovered that BK activates extracellular signal-regulated protein kinase 1 and 2 (ERK) in HEK293 cells. Thus, we hypothesized that HEK293 cells possess endogenous BK receptors. RT-PCR demonstrated the presence of mRNAs for BK B1 and BK B2 receptors in HEK293 cells. Western blotting with BK B1 and BK B2 receptor antibodies confirmed this result at the protein level. To establish that BK receptors are functional, we employed fluorescent measurements of intracellular Ca2+, measured changes in extracellular acidification rate (ECAR) as a reflection of the Na+/H+ exchange (NHE) with a Cytosensor™ microphysiometer, and assessed ERK activation by Western blotting with a phospho-specific ERK antibody. Exposure of HEK293 cells to BK produced a concentration-dependent rise in intracellular Ca2+ (EC50 = 36.5 ± 8.0 × 10−9 M), a rapid increase in tyrosine phosphorylation of ERK (EC50 = 9.8 ± 0.4 × 10−9 M), and elevation in ECAR by ∼20%. All of these signals were blocked by HOE-140 (B2 receptor antagonist) but not by des-Arg10-HOE-140 (B1 receptor antagonist). We conclude that HEK293 cells express endogenous functional BK B2 receptors, which couple to the mobilization of intracellular Ca2+, increases in ECAR and increases in ERK phosphorylation." @default.
• W2006793940 created "2016-06-24" @default.
• W2006793940 creator A5020868521 @default.
• W2006793940 creator A5021338724 @default.
• W2006793940 creator A5033763744 @default.
• W2006793940 creator A5045217697 @default.
• W2006793940 creator A5066493268 @default.
• W2006793940 date "2009-01-01" @default.
• W2006793940 modified "2023-10-14" @default.
• W2006793940 title "Identification of functional bradykinin B2 receptors endogenously expressed in HEK293 cells" @default.
• W2006793940 cites W1505408758 @default.
• W2006793940 cites W1793395791 @default.
• W2006793940 cites W1925704923 @default.
• W2006793940 cites W1938379229 @default.
• W2006793940 cites W1981719698 @default.
• W2006793940 cites W1994482946 @default.
• W2006793940 cites W2011602348 @default.
• W2006793940 cites W2021495235 @default.
• W2006793940 cites W2024894359 @default.
• W2006793940 cites W2033171480 @default.
• W2006793940 cites W2036213823 @default.
• W2006793940 cites W2044296969 @default.
• W2006793940 cites W2068006705 @default.
• W2006793940 cites W2077060302 @default.
• W2006793940 cites W2083493190 @default.
• W2006793940 cites W2087552678 @default.
• W2006793940 cites W2097812689 @default.
• W2006793940 cites W2098093331 @default.
• W2006793940 cites W2101093047 @default.
• W2006793940 cites W2105429058 @default.
• W2006793940 cites W2114458975 @default.
• W2006793940 cites W2115978962 @default.
• W2006793940 cites W2124665478 @default.
• W2006793940 cites W2133636515 @default.
• W2006793940 cites W2139663228 @default.
• W2006793940 cites W2146469259 @default.
• W2006793940 cites W2156440761 @default.
• W2006793940 cites W2172079406 @default.
• W2006793940 cites W2182987870 @default.
• W2006793940 cites W2183177478 @default.
• W2006793940 cites W2191700886 @default.
• W2006793940 cites W2272561101 @default.
• W2006793940 cites W2419626516 @default.
• W2006793940 doi "https://doi.org/10.1016/j.bcp.2008.09.027" @default.
• W2006793940 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2645039" @default.
• W2006793940 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18938142" @default.
• W2006793940 hasPublicationYear "2009" @default.
• W2006793940 type Work @default.
• W2006793940 sameAs 2006793940 @default.
• W2006793940 citedByCount "23" @default.
• W2006793940 countsByYear W20067939402012 @default.
• W2006793940 countsByYear W20067939402013 @default.
• W2006793940 countsByYear W20067939402014 @default.
• W2006793940 countsByYear W20067939402016 @default.
• W2006793940 countsByYear W20067939402017 @default.
• W2006793940 countsByYear W20067939402018 @default.
• W2006793940 crossrefType "journal-article" @default.
• W2006793940 hasAuthorship W2006793940A5020868521 @default.
• W2006793940 hasAuthorship W2006793940A5021338724 @default.
• W2006793940 hasAuthorship W2006793940A5033763744 @default.
• W2006793940 hasAuthorship W2006793940A5045217697 @default.
• W2006793940 hasAuthorship W2006793940A5066493268 @default.
• W2006793940 hasBestOaLocation W20067939402 @default.
• W2006793940 hasConcept C104317684 @default.
• W2006793940 hasConcept C135285700 @default.
• W2006793940 hasConcept C153911025 @default.
• W2006793940 hasConcept C170493617 @default.
• W2006793940 hasConcept C174510640 @default.
• W2006793940 hasConcept C185592680 @default.
• W2006793940 hasConcept C28406088 @default.
• W2006793940 hasConcept C54009773 @default.
• W2006793940 hasConcept C54355233 @default.
• W2006793940 hasConcept C55493867 @default.
• W2006793940 hasConcept C57074206 @default.
• W2006793940 hasConcept C62478195 @default.
• W2006793940 hasConcept C79879829 @default.
• W2006793940 hasConcept C81885089 @default.
• W2006793940 hasConcept C85265743 @default.
• W2006793940 hasConcept C86803240 @default.
• W2006793940 hasConcept C95444343 @default.
• W2006793940 hasConceptScore W2006793940C104317684 @default.
• W2006793940 hasConceptScore W2006793940C135285700 @default.
• W2006793940 hasConceptScore W2006793940C153911025 @default.
• W2006793940 hasConceptScore W2006793940C170493617 @default.
• W2006793940 hasConceptScore W2006793940C174510640 @default.
• W2006793940 hasConceptScore W2006793940C185592680 @default.
• W2006793940 hasConceptScore W2006793940C28406088 @default.
• W2006793940 hasConceptScore W2006793940C54009773 @default.
• W2006793940 hasConceptScore W2006793940C54355233 @default.
• W2006793940 hasConceptScore W2006793940C55493867 @default.
• W2006793940 hasConceptScore W2006793940C57074206 @default.
• W2006793940 hasConceptScore W2006793940C62478195 @default.
• W2006793940 hasConceptScore W2006793940C79879829 @default.
• W2006793940 hasConceptScore W2006793940C81885089 @default.
• W2006793940 hasConceptScore W2006793940C85265743 @default.
• W2006793940 hasConceptScore W2006793940C86803240 @default.
• W2006793940 hasConceptScore W2006793940C95444343 @default.
• W2006793940 hasIssue "2" @default.

• next