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• W2006842456 startingPage "13115" @default.
• W2006842456 abstract "Intracellular pH is an important indicator for cellular metabolism and pathogenesis. pH sensing in living cells has been achieved using a number of synthetic organic dyes and genetically expressible sensor proteins, even allowing the specific targeting of intracellular organelles. Ideally, a class of genetically encodeable sensors need to cover relevant cellular pH ranges. We present a FRET-based pH sensor platform, based on the pH modulation of YFP acceptor fluorophores in a fusion construct with ECFP. The concurrent loss of the overlap integral upon acidification results in a proportionally reduced FRET coupling. The readout of FRET over the sensitized YFP fluorescence lifetime yields a highly sensitive and robust pH measurement that is self-calibrated. The principle is demonstrated in the existing high-efficiency FRET fusion Cy11.5, and tunability of the platform design is demonstrated by genetic alteration of the pH sensitivity of the acceptor moiety." @default.
• W2006842456 created "2016-06-24" @default.
• W2006842456 creator A5012945538 @default.
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• W2006842456 date "2008-11-13" @default.
• W2006842456 modified "2023-09-26" @default.
• W2006842456 title "pHlameleons: A Family of FRET-Based Protein Sensors for Quantitative pH Imaging" @default.
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• W2006842456 doi "https://doi.org/10.1021/bi8009482" @default.
• W2006842456 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19007185" @default.
• W2006842456 hasPublicationYear "2008" @default.
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