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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2006907494> ?p ?o ?g. }

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• W2006907494 abstract "A cDNA sequence coding for a human granulocyte-macrophage colony-stimulating factor has been isolated from cDNA libraries prepared from mRNA derived from concanavalin A-activated human T-cell clones. The libraries constructed in the pcD vector system were screened by transfecting COS-7 monkey cells with DNA pools to express the products encoded by full-length cDNA inserts. By assaying the cell supernatants, we identified clones encoding a factor that stimulates the formation of granulocyte and macrophage colonies from human progenitor cells. These results demonstrate that identification of full-length cDNAs for many colony-stimulating factors may be achieved entirely on the basis of detecting the functional polypeptide produced in mammalian cells." @default.
• W2006907494 created "2016-06-24" @default.
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• W2006907494 date "1985-07-01" @default.
• W2006907494 modified "2023-10-13" @default.
• W2006907494 title "Isolation of cDNA for a human granulocyte-macrophage colony-stimulating factor by functional expression in mammalian cells." @default.
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• W2006907494 doi "https://doi.org/10.1073/pnas.82.13.4360" @default.
• W2006907494 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/390413" @default.
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