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- W2006957037 abstract "Abstract A split-flow enzyme thermistor for biosensing of cholesterol is described. The device measures the heats of oxidation and decomposition evolved when a cholesterol sample is passed through a column containing three kinds of enzymes (cholesterol esterase, cholesterol oxidase and catalase) covalently co-immobilized onto porous glass beads. The calibration graph for standard cholesterol is linear up to 400 mg/dl for 50 μl samples. The relative standard deviation for 200 mg/dl cholesterol is 1.5% (n = 30). The assay takes 3 min or less. The proposed method is applied to the determination of cholesterol in human blood sera and compared with a conventional spectrophotometric method." @default.
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- W2006957037 date "1991-08-01" @default.
- W2006957037 modified "2023-10-02" @default.
- W2006957037 title "Biosensing of cholesterol with use of a split-flow enzyme thermistor" @default.
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- W2006957037 doi "https://doi.org/10.1016/0925-4005(91)80257-k" @default.
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