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• W2006958151 abstract "<i>Introduction:</i> Hepatocyte growth factor (HGF) is a target of gene therapy for renal fibrosis. The aim of this study was to establish a human HGF gene expression system that is regulated by tetracycline (Tet) in normal rat kidney tubular epithelial cells (NRK52E cells). <i>Materials and Methods:</i> The plasmids pTet-on, pBI-L-HGF and pTK-Hyg were transfected sequentially into NRK52E cells using Lipofectamine 2000. The expression of HGF gene was measured, and the activity of expressed HGF was detected. <i>Results:</i> A clone of pBI-L-HGF/NRK52E cells showing strong reaction to doxycycline (Dox) was selected using a luciferase reporter assay system. The expression of both HGF mRNA and protein was significantly higher (both p < 0.01) in the Dox group than that in the control group. Furthermore, the bioactivity of expressed HGF was confirmed in the assay. <i>Conclusions:</i> A Tet-regulated human HGF gene expression system in NRK52E cells has been established. This cell line may prove useful for gene therapy against renal fibrosis." @default.
• W2006958151 created "2016-06-24" @default.
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• W2006958151 date "2010-01-01" @default.
• W2006958151 modified "2023-10-16" @default.
• W2006958151 title "Developing a System for Regulating Expression of Human Hepatocyte Growth Factor Using Tetracycline in NRK52E Cells" @default.
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• W2006958151 doi "https://doi.org/10.1159/000314956" @default.
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