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- W2007008082 abstract "The stimulation by phytohemagglutinin (PHA) of DNA synthesis in cultured blood lymphocytes of guinea pig was markedly inhibited by addition of leupeptin, a well-characterized, powerful protease inhibitor of tripeptide nature. About 30 to 40 per cent inhibition was observed at 40 μg/ml of leupeptin when leupeptin was added 30 min prior to or together with PHA. Per cent inhibition by the appropriate amount of leupeptin was proportional to the amount of PHA added in the range of 0.6 to 3.0 μg of PHA at which the per cent inhibition reached maximum. This inhibitory effect of leupeptin on PHA stimulation was abolished when the lymphocytes were preincubated with PHA for more than 10 min before addition of leupeptin or preincubated with leupeptin for more than 60 min prior to PHA addition." @default.
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- W2007008082 date "1973-05-01" @default.
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- W2007008082 title "Involvement of proteolytic activity in early events in lymphocyte transformation by phytohemagglutinin" @default.
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- W2007008082 doi "https://doi.org/10.1016/0006-291x(73)90750-x" @default.
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